Maturation of bone marrow-derived dendritic cells by a novel beta-glucan purified from Paenibacillus polymyxa JB115.
10.4142/jvs.2011.12.2.187
- Author:
Eun Ju KO
1
;
Yun Young BYON
;
Youngheun JEE
;
Taekyun SHIN
;
Seung Chun PARK
;
Tae Wook HAHN
;
Hong Gu JOO
Author Information
1. College of Veterinary Medicine, Jeju National University, Jeju 690-756, Korea. jooh@jejunu.ac.kr
- Publication Type:Brief Communication ; Research Support, Non-U.S. Gov't
- Keywords:
dendritic cells;
JB115;
maturation;
Paenibacillus polymyxa;
beta-glucan
- MeSH:
Animals;
Bone Marrow Cells/cytology/*drug effects/*immunology;
Cell Survival/drug effects/*immunology;
Dendritic Cells/cytology/*drug effects/*immunology;
Flow Cytometry;
Immunophenotyping/methods;
Interleukin-12/analysis/immunology;
Mice;
Mice, Inbred BALB C;
Nitric Oxide/analysis/immunology;
Paenibacillus/*chemistry;
Tumor Necrosis Factor-alpha/analysis/immunology;
beta-Glucans/isolation & purification/*pharmacology
- From:Journal of Veterinary Science
2011;12(2):187-189
- CountryRepublic of Korea
- Language:English
-
Abstract:
We investigated the immunostimulatory effects of a novel beta-glucan purified from Paenibacillus (P.) polymyxa JB115 on bone marrow-derived dendritic cells (DCs), a type of potent antigen-presenting cells. beta-glucan isolated from P. polymyxa JB115 enhanced the viability and induced the maturation of DCs. beta-glucan markedly increased the cytokine production of DCs and surface expression of DC markers. In addition, DCs treated with beta-glucan showed a higher capacity to stimulate allogeneic spleen cell proliferation compared to those treated with medium alone. These results demonstrate the effect of beta-glucan on DC maturation and may increase the use of beta-glucan.
