A monoclonal antibody to cell surface antigen of human thymic epithelial cell.
- Author:
Doo Hyun CHUNG
1
;
Young Mee BAE
;
Hee Young SHIN
;
Hyo Seop AHN
;
Hyung Geun SONG
;
Wwon Seo PARK
;
Seong Hoe PARK
;
Sang Kook LEE
Author Information
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- MeSH: Animals; Antibodies, Monoclonal/biosynthesis/*immunology; Antibody Specificity; Antigens, Surface/*immunology; Epithelium/immunology; Fluorescent Antibody Technique; Humans; Immunoenzyme Techniques; Immunoglobulin G/immunology; Immunoglobulin Isotypes/immunology; Mice; Mice, Inbred BALB C; Neoplasms/immunology; Thymoma/immunology; Thymus Gland/*immunology; Thymus Neoplasms/immunology; Tumor Cells, Cultured
- From:Journal of Korean Medical Science 1994;9(1):47-51
- CountryRepublic of Korea
- Language:English
- Abstract: The cell surface molecule identified by a monoclonal antibody(TE-1) to human thymic epithelial cell showed the specificity for thymic epithelial cells of both the cortex and medulla. TE-1 reacted with the epithelial cells of normal thymus and thymoma in fresh frozen tissues. The antigen recognized by TE-1 was mostly confined to the cell surface membrane and arranged in reticular network with long processes between thymocytes. On immunohistochemical analysis, TE-1 did not recognize normal epithelial cells of the uterine cervix, skin and stomach, and neoplastic cells of squamous cell carcinoma and gastric adenocarcinoma, all of which were stained with anti-cytokeratin monoclonal antibody. Among the tumor cell lines tested with flow cytometry, most of epithelial and all of hematopoietic cell origin were not labeled with TE-1. In summary, TE-1 appears to be a monoclonal antibody against a surface antigen of human thymic epithelial cell that is immunohistologically different from known epithelial cell surface antigens reported so far.
