MIF Test Using Lens Protein as Antigen.
- Author:
Hum CHUNG
1
Author Information
1. Department of Ophthalmology, College of Medicine, Seoul National University, Seoul, Korea.
- Publication Type:Original Article
- MeSH:
Agar;
Animals;
Endophthalmitis;
Exudates and Transudates;
Freund's Adjuvant;
Guinea Pigs;
Hypersensitivity;
Immunization;
Mineral Oil;
Paraffin;
Peritoneal Cavity;
Skin;
Skin Tests;
Staphylococcal Protein A*
- From:Journal of the Korean Ophthalmological Society
1976;17(4):415-419
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
This experiment was performed to demonstrate that the cell-mediated hypersensitivity plays an important role in the development of the so-called endophthalmitis phaooanaphylactica. Fifteen albino guinea pigs were immunized with sclubte proteins of bovine lens with complete Freund's adjuvant. The immunizations were repeated 4 times at 5 days intervals. 5 days after the last injection, the skin test was performed with soluble lens protein, and liquid paraffin was injected into the peritoneal cavity. 7 days after paraffin injection, the peritoneal exudate cells were harvested, and were allowed to migrate on agar with and without antigen according to Salvin- Niahio method. All of them shows positive skin reaction and significant inhibition of migration under the presence of the antigen.
