Genotyping of a Korean isolate of Toxoplasma gondii by multilocus PCR-RFLP and microsatellite analysis.
10.3347/kjp.2008.46.2.105
- Author:
Juan Hua QUAN
1
;
Tae Yun KIM
;
In Uk CHOI
;
Young Ha LEE
Author Information
1. Department of Infection Biology, College of Medicine, Reseach Institute for Medical Science, Chungnam National University, Daejeon 301-747, Korea. yhalee@cnu.ac.kr
- Publication Type:Original Article ; Comparative Study ; Research Support, Non-U.S. Gov't
- Keywords:
Toxoplasma gondii;
Korean isolate;
genotype
- MeSH:
Animals;
Base Sequence;
Cell Line;
DNA, Protozoan/genetics;
Genotype;
Korea;
Mice;
*Microsatellite Repeats;
Molecular Sequence Data;
Polymerase Chain Reaction;
*Polymorphism, Restriction Fragment Length;
Sequence Alignment;
Toxoplasma/classification/*genetics/*isolation & purification
- From:The Korean Journal of Parasitology
2008;46(2):105-108
- CountryRepublic of Korea
- Language:English
-
Abstract:
Although the Korean isolate KI-1 of Toxoplasma gondii has been considered to be a virulent type I lineage because of its virulent clinical manifestations, its genotype is unclear. In the present study, genotyping of the KI-1 was performed by multilocus PCR-RFLP and microsatellite sequencing. For 9 genetic markers (c22-8, c29-2, L358, PK1, SAG2, SAG3, GRA6, BTUB, and Apico), the KI-1 and RH strains exhibited typical PCR-RFLP patterns identical to the type I strains. DNA sequencing of tandem repeats in 5 microsatellite markers (B17, B18, TUB2, W35, and TgM-A) of the KI-1 also revealed patterns characteristic of the type I. These results provide strong genetic evidence that KI-1 is a type I lineage of T. gondii.
