Surface characteristics and osteoblastic cell response of alkali-and heat-treated titanium-8tantalum-3niobium alloy.
10.5051/jpis.2012.42.6.248
- Author:
Bo Ah LEE
1
;
Choong Hee KANG
;
Mong Sook VANG
;
Young Suk JUNG
;
Xing Hui PIAO
;
Ok Su KIM
;
Hyun Ju CHUNG
;
Young Joon KIM
Author Information
1. Department of Periodontology, Dental Research Institute, Chonnam National University School of Dentistry, Gwangju, Korea. youngjun@chonnam.ac.kr
- Publication Type:Original Article
- Keywords:
Alkaline phosphatase;
Cell adhesion;
Cell proliferation;
Surface properties;
Titanium alloy
- MeSH:
Alkaline Phosphatase;
Alloys;
Animals;
Cell Adhesion;
Cell Proliferation;
Microscopy, Atomic Force;
Microscopy, Electron, Scanning;
Osteoblasts;
Oxygen;
Photoelectron Spectroscopy;
Rats;
Spectrum Analysis;
Surface Properties;
Titanium
- From:Journal of Periodontal & Implant Science
2012;42(6):248-255
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: The aim of the present study was to evaluate the biological response of alkali- and heat-treated titanium-8tantalum-3niobium surfaces by cell proliferation and alkaline phosphatase (ALP) activity analysis. METHODS: Commercial pure titanium (group cp-Ti) and alkali- and heat-treated titanium-8tantalum-3niobium (group AHT) disks were prepared. The surface properties were evaluated using scanning electron microscopy, energy dispersed spectroscopy and X-ray photoelectron spectroscopy (XPS). The surface roughness was evaluated by atomic force microscopy and a profilometer. The contact angle and surface energy were also analyzed. The biological response of fetal rat calvarial cells on group AHT was assessed by cell proliferation and ALP activity. RESULTS: Group AHT showed a flake-like morphology microprofile and dense structure. XPS analysis of group AHT showed an increased amount of oxygen in the basic hydroxyl residue of titanium hydroxide groups compared with group cp-Ti. The surface roughness (Ra) measured by a profilometer showed no significant difference (P>0.05). Group AHT showed a lower contact angle and higher surface energy than group cp-Ti. Cell proliferation on group AHT surfaces was significantly higher than on group cp-Ti surfaces (P<0.05). In comparison to group cp-Ti, group AHT enhanced ALP activity (P<0.05). CONCLUSIONS: These results suggest that group AHT stimulates osteoblast differentiation.
