Effects of Intronic and Exonic Polymorphisms of Paraoxonase 1 (PON1) Gene on Serum PON1 Activity in a Korean Population.
10.3346/jkms.2011.26.6.720
- Author:
Sang Yong EOM
1
;
Yun Sik KIM
;
Chung Jong LEE
;
Chul Ho LEE
;
Yong Dae KIM
;
Heon KIM
Author Information
1. Department of Preventive Medicine and Medical Research Institute, College of Medicine, Chungbuk National University, Cheongju, Korea. kimheon@cbu.ac.kr
- Publication Type:Original Article
- Keywords:
Paraoxonase 1 (PON1);
Genetic Polymorphism;
Linkage Disequilibrium;
serum PON1 activity
- MeSH:
Aged;
Alleles;
Aryldialkylphosphatase/blood/*genetics;
Asian Continental Ancestry Group/*genetics;
Exons;
Female;
Gene Frequency;
Genotype;
Humans;
Introns;
Linkage Disequilibrium;
Lipoproteins, HDL/blood;
Male;
Middle Aged;
*Polymorphism, Genetic;
Polymorphism, Single Nucleotide;
Regression Analysis;
Republic of Korea;
Smoking;
Triglycerides/blood
- From:Journal of Korean Medical Science
2011;26(6):720-725
- CountryRepublic of Korea
- Language:English
-
Abstract:
Paraoxonase 1 (PON1) hydrolyzes a number of toxic organophosphorous compounds and reduces lipid peroxide accumulation, and PON1 genetic polymorphisms in the coding region modulate serum PON1 activity. In this study, we investigated the association between 3 polymorphisms of PON1 located in intron 5 (17899insdelTT and 17974CT) and exon 6 (192QR) and serum PON1 activity. The genetic polymorphisms and serum activity of PON1 were analyzed in 153 healthy Koreans by using a direct sequencing assay and spectrophotometric method, respectively. A significant linkage disequilibrium (LD) was observed between all tested single nucleotide polymorphisms, with the strongest LD observed between 17899insdelTT and 192QR (D' = 0.984). The 17899insdelTT, 17974CT and 192QR genetic polymorphisms were associated with significant differences in serum paraoxonase activity. In multiple regression analyses, smoking, triglyceride level, high-density lipoprotein (HDL) level, and the 17899insdelTT and 192QR genetic polymorphisms were significant determinants of serum paraoxonase activity, while age, smoking, triglyceride level, HDL level, and the 192QR genetic polymorphism were significant determinants of serum arylesterase activity. These results suggest that although the 192QR genetic polymorphism in the coding region of PON1 is primarily associated with serum PON1 activity, the intronic polymorphisms are also involved in serum PON1 activity, and this association may be mediated by LD.
