Sequence Analysis of the Human Tyrosinase Gene in Korean.
10.5021/ad.1995.7.1.34
- Author:
Kyoung Chan PARK
;
Kyu Han KIM
- Publication Type:Original Article
- Keywords:
Nucleotide sequence;
Tyrosinase gene
- MeSH:
Albinism, Oculocutaneous;
Amino Acids;
Base Sequence;
Clinical Coding;
DNA;
Exons;
Humans*;
Molecular Weight;
Monophenol Monooxygenase*;
Population Characteristics;
Sequence Analysis*
- From:Annals of Dermatology
1995;7(1):34-38
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGOUND: Because heterogeneity of the human tyrosinase gene has been reported, it is of vital significance to know the correct nucleotide sequence of tyrosinase in Koreans in order to study the molecular basis of oculocutaneous albinism. OBJECTIVE: This study was done to analyze the nucleotide sequence of tyrosinase gene in Koreans. METHODS: All exons were amplified from genomic DNAs and sequenced directly from three healthy Koreans. RESULTS: The nucleotide sequence of the tyrosinase gene in Koreans encode 511 amino acids with a molecular weight of 58,000. CONCLUSION: The nucleotide sequence of coding region is the same with that of pHTrl(Shibahara et al. Tohoku J Exp Med 156: 403-414, 1988). As we couldn't observe any polymorphisms in Koreans these results suggested the homogeneity of Korean people.