The Effect of Sil-Select and Percoll on the Ultrastructure of Spermatozoa.
- Author:
Sung Hoon KIM
;
Chung Hoon KIM
;
Eun Ju PARK
;
Seok Ho HONG
;
Hee Young NAH
;
Jung Hee KIM
;
Hyun Jin EM
;
Sook Hee KIM
;
Hee Dong CHAE
;
Byung Moon KANG
;
Joo Ryung HUH
;
Yoon Seok CHANG
- Publication Type:Original Article
- Keywords:
Percoll;
Sil-Select;
Transmission electron microscopy;
Strict criteria
- MeSH:
Acrosome;
Humans;
Male;
Microscopy;
Microscopy, Electron, Transmission;
Mitochondria;
Semen;
Spermatozoa*
- From:Korean Journal of Obstetrics and Gynecology
2000;43(7):1246-1252
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: We performed this study to evaluate the effects of Sil-Select and Percoll in sperm preparation. METHODS: Semen samples of 22 patients with normal sperm parameters by WHO criteria were divided into two equal parts and prepared with Percoll and Sil-Select. After completion of semen preparation procedures with Percoll and Sil-Select, sperm concentration, motility and morphology using strict criteria were evaluated in each group and all semen samples were fixed and stained for transmission electron microscopy(TEM). RESULTS: There were no significant diffrences in sperm concentration, percentage of motile spermatozoa and percentage of normal spermatozoa in morphology evaluation using strict criteria under light microscopy between Percoll and Sil-Select-treated groups. However, the percentage of normal shape and position of acrosome, and normal helix assembly of mitochondria under TEM were significantly higher in the Sil-Select-treated group compared to Percoll-treated group (p < 0.001, p < 0.001, p < 0.001). CONCLUSIONS: These data demonstrate that Sil-Select is less detrimental to the acrosome and mitochondria of spermatozoa in sperm preparation compared to Percoll.
