Percentage of motile spermatozoa at 22 hours after swim-up procedure: An indicator for intracytoplasmic sperm injection?.
10.5653/cerm.2016.43.3.157
- Author:
Taketo INOUE
1
;
Yukiko YONEZAWA
;
Hironobu SUGIMOTO
;
Mikiko UEMURA
;
Yuri ONO
;
Junji KISHI
;
Nobuyuki EMI
;
Yoshiyuki ONO
Author Information
1. Ono Ladies Clinic, Ono, Japan. taketoein@yahoo.co.jp
- Publication Type:Original Article
- Keywords:
Fertilization in vitro;
Insemination;
Sperm injections;
intracytoplasmic;
Spermatozoa
- MeSH:
Blastocyst;
Embryonic Development;
Female;
Fertilization;
Fertilization in Vitro;
Incidence;
Insemination;
Live Birth;
Pregnancy;
Prospective Studies;
Sperm Injections, Intracytoplasmic*;
Spermatozoa*
- From:Clinical and Experimental Reproductive Medicine
2016;43(3):157-163
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVE: The decision to use in vitro fertilization (IVF), intracytoplasmic sperm injection (ICSI), or split insemination (IVF-ICSI) in the first cycle is based on the number of motile sperm. Hence, total fertilization failure (TFF) often occurs during IVF cycles, despite normozoospermia. To investigate whether the cumulative motile swim-up spermatozoa percentage at 22 hours post-insemination (MSPPI) is an indicator for ICSI, we analyzed TFF, fertilization, blastocyst development, chemical pregnancy, clinical pregnancy, and live birth rates. METHODS: This prospective study was performed using data obtained from 260 IVF cycles. At 22 hours after insemination, the remaining swim-up spermatozoa were observed and divided into six groups according to MSPPI (<10%, 10% to <30%, 30% to <50%, 50% to <70%, 70% to <90%, and 90% to 100%). RESULTS: Regardless of the ejaculated motile sperm concentration (0.6–280×106/mL motile spermatozoa), the incidence of TFF significantly increased when MSPPI was <10%, and the fertilization rate significantly decreased when MSPPI was <30%. We found that cumulative MSPPI correlated with the cumulative fertilization rate (Spearman correlation, 0.508, p<0.001). Regarding embryo development, we observed no significant differences in the rates of blastocyst development, chemical pregnancy, clinical pregnancy, or live birth among all groups. CONCLUSION: Our findings suggest that MSPPI is a viable indicator for split IVF-ICSI and ICSI. Taken together, by employing the MSPPI test in advance before IVF, ICSI, or split IVF-ICSI cycles, unnecessary split IVF-ICSI and ICSI may be avoided.
