Genotype and animal infectivity of a human isolate of Cryptosporidium parvum in the Republic of Korea.
10.3347/kjp.2004.42.2.85
- Author:
Sang Mee GUK
;
Tai Soon YONG
;
Soon Jung PARK
;
Jae Hwan PARK
;
Jong Yil CHAI
- Publication Type:Brief Communication ; Research Support, Non-U.S. Gov't
- Keywords:
Cryptosporidium parvum;
oocyst;
human;
calf;
mouse;
genotype
- MeSH:
Animals;
Cattle;
Child;
Cryptosporidiosis/microbiology;
Cryptosporidium parvum/*genetics/immunology;
Diarrhea/parasitology;
Feces/parasitology;
Genotype;
Human;
Korea;
Male;
Mice;
Mice, Inbred BALB C;
Mice, Inbred C57BL;
Mice, Inbred ICR;
Oocysts;
Polymerase Chain Reaction;
Support, Non-U.S. Gov't;
Zoonoses/parasitology
- From:The Korean Journal of Parasitology
2004;42(2):85-89
- CountryRepublic of Korea
- Language:English
-
Abstract:
Cryptosporidium parvum oocysts were isolated from a child suffering from acute gastroenteritis and successfully passaged in a calf and mice (designated hereafter SNU-H1) in the Republic of Korea; its molecular genotype has been analyzed. The GAG microsatellite region was amplified by a polymerase chain reaction (PCR), with a 238 base pair product, which is commonly displayed in C. parvum. The isolate was shown to be a mixture of the genotypes 1 (anthroponotic) and 2 (zoonotic). To study its infectivity in animals, 2 calves and 3 strains of mice were infected with the SNU-H1; in these animals, the propagation of both genotypes was successful. In immunosuppressed (ImSP) BALB/c and C57BL/6 mice the number of oocysts decreased after day 10 post-infection (PI) ; but in ImSP ICR mice, they remained constant until day 27 PI. The results show that both the C. parvum genotypes 1 and 2 can be propagated in calves and ImSP mice.
