Expression Profiling of Calcium Induced Genes in Cultured Human Keratinocytes.
10.3346/jkms.2010.25.4.619
- Author:
Jung Suk LEE
1
;
Mi Rang KIM
;
Nam Soon KIM
;
Yong Sung KIM
;
Jun Mo YANG
;
Ah Young CHO
;
Young LEE
;
Chang Deok KIM
;
Jeung Hoon LEE
Author Information
1. Department of Dermatology and Research Institute for Medical Sciences, School of Medicine, Chungnam National University, Daejeon, Korea. jhoon@cnu.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Keratinocyte Differentiation;
Calcium;
Normalized Library;
Gene Expression Profiling
- MeSH:
Calcium/*metabolism;
Cells, Cultured;
Chromosome Mapping;
Chromosomes, Human;
Expressed Sequence Tags;
Gene Expression Profiling;
*Gene Expression Regulation;
Gene Library;
Humans;
Keratinocytes/cytology/*physiology;
Molecular Sequence Data;
Oligonucleotide Array Sequence Analysis
- From:Journal of Korean Medical Science
2010;25(4):619-625
- CountryRepublic of Korea
- Language:English
-
Abstract:
Terminal differentiation of skin keratinocytes is a vertically directed multi-step process that is tightly controlled by the sequential expression of a variety of genes. To examine the gene expression profile in calcium-induced keratinocyte differentiation, we constructed a normalized cDNA library using mRNA isolated from these calcium-treated keratinocytes. After sequencing about 10,000 clones, we were able to obtain 4,104 independent genes. They consisted of 3,699 annotated genes and 405 expressed sequence tags (ESTs). Some were the genes involved in constituting epidermal structures and others were unknown genes that are probably associated with keratinocytes. In particular, we were able to identify genes located at the chromosome 1q21, the locus for the epidermal differentiation complex, and 19q13.1, another probable locus for epidermal differentiation-related gene clusters. One EST located at the chromosome 19q13.1 showed increased expression by calcium treatment, suggesting a novel candidate gene relevant to keratinocyte differentiation. These results demonstrate the complexity of the transcriptional profile of keratinocytes, providing important clues on which to base further investigations of the molecular events underlying keratinocyte differentiation.
