Immunohistochemical Study of Proliferative Vitreoretinal Membranes.
- Author:
Sang In KWAK
1
;
Se Oh OH
;
Hum CHUNG
;
Jaeheung LEE
Author Information
1. Department of Ophthalmology, College of Medicine, Seoul National University, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Cellular component;
Immunohistochemical study;
Proliferative diabetic retinopathy;
Proliferative vitreoretinopathy;
Proliferative vitreoretinal membrane
- MeSH:
Antibodies, Monoclonal;
Diabetic Retinopathy;
Fibroblasts;
Immunoenzyme Techniques;
Keratins;
Membranes*;
Neuroglia;
Retinaldehyde;
Vimentin;
Vitreoretinopathy, Proliferative
- From:Journal of the Korean Ophthalmological Society
1992;33(4):349-356
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
To identify the cellular components of proliferative vitreoretinal membranes. 11 proliferative vitreoretinal membranes which were surgically obtained from 7 eyes with proliferative vitreo retinopathy (PVR) and 4 eyes with proliferative diabetic retinopathy (PDR) were stained with monoclonal antibodies against cytokeratin, glial fibrillary acidic protein(GFAP), or vimentin using immunoperoxidase technique (ABC method). In PVR membranes, mean cell positivities for cytokeratin, GFAP and vimentin were 48%, 1% and 92%, respectively and in PDR membranes, 0%, 5% and 93%, respectively. The above results suggest that retinal pigment epithelial celIs and fibroblasts are major cellular components of PVR membranes, and mesenchymal cells are major cellular components and glial cells minor cellular components of PDR membranes.
