Evaluation of Phase-Contrast Microscopic Imaging with Synchrotron Radiation in the Diagnosis of Breast Cancer and Differentiation of Various Breast Diseases: Preliminary Results.
10.4048/jbc.2010.13.4.349
- Author:
Young Ju JEONG
1
;
Jin Gu BONG
;
Hong Tae KIM
;
Jong Ki KIM
;
Hoon Kyu OH
;
Jung Yun HUANG
;
Sung Hwan PARK
Author Information
1. Department of Surgery, Catholic University of Daegu School of Medicine, Daegu, Korea. shwpark@cu.ac.kr
- Publication Type:Original Article
- Keywords:
Breast;
Phase-contrast microscopy;
Synchrotron radiation
- MeSH:
Breast;
Breast Diseases;
Breast Neoplasms;
Carcinoma, Ductal;
Carcinoma, Intraductal, Noninfiltrating;
Discrimination (Psychology);
Microscopy;
Microscopy, Phase-Contrast;
Synchrotrons
- From:Journal of Breast Cancer
2010;13(4):349-356
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: A significant improvement of imaging using synchrotron radiation (SR) is obtained by introducing phase-contrast technique. This technique provides greatly enhanced contrast and good soft tissue discrimination with high spatial resolution. The aim of this study was to observe microstructures of pathologic breast specimens including invasive breast cancer using phase-contrast technique with SR and to evaluate the feasibility of phase-contrast imaging in clinical application. METHODS: Phase-contrast microscopic image of normal breast tissue and the images of various breast diseases such as fibrocystic change, ductal carcinoma in situ, invasive ductal carcinoma, Paget's disease were obtained using hard X-ray microscopy with an 11.1 keV monochromatic beam from SR source and CsI (TI) scintillation crystal. Zernike phase-shifter was adapted for phase-contrast hard X-ray microscopy. The visual image was magnified 20 times by microscopic objective lens and captured using a full frame charge-coupled device camera. Obtained images were compared with corresponding histopathologic findings in the optical microscopy. RESULTS: The SR images of various breast diseases were obtained with a good contrast and high visibility by phase-contrast technique. It was possible to observe the microstructures with high spatial resolution down to the micron region. The characteristic features of each disease were consistent with the histopathologic findings of corresponding sample and the images of breast cancer and the other diseases were distinct from each other. CONCLUSION: Using phase-contrast technique, SR images of various breast diseases including breast cancer were obtained. These images were comparable with standard histopathologic findings and showed different features for each disease. The results suggest that phase-contrast microscopic imaging with SR has potential as a diagnostic tool and also its clinical application is feasible, especially in breast imaging.
