Mutational Analysis of Proapoptotic Bcl-2 Family Members in Gastric Carcinomas.
10.5230/jkgca.2003.3.2.84
- Author:
Nam Jin YOO
1
;
Jong Woo LEE
;
Young Hwa SOUNG
;
Hong Sug KIM
;
Won Sang PARK
;
Jung Young LEE
;
Sug Hyung LEE
Author Information
1. Department of Pathology, College of Medicine, The Catholic University of Korea, Seoul, Korea. suhulee@catholic.ac.kr
- Publication Type:Original Article
- Keywords:
Gastric carcinoma;
BAD;
BIM;
BIK;
Bcl-G;
Apoptosis;
Mutation
- MeSH:
Adenocarcinoma;
Apoptosis;
Base Sequence;
Carcinogenesis;
Clinical Coding;
Humans;
Polymerase Chain Reaction;
Polymorphism, Single-Stranded Conformational;
Sequence Analysis, DNA
- From:Journal of the Korean Gastric Cancer Association
2003;3(2):84-87
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Evidence exists that dysregulation of Bcl-2 family members is involved in the pathogenesis of cancer development. The aim of this study was to explore whether the somatic mutation of proapoptotic Bcl-2 member genes, one of the mechanisms that prolong the survival of cancer cells, is involved in gastric carcinogenesis. MATERIALS AND METHODS: In the current study, to detect somatic mutations of the DNA sequences encoding the Bcl-2 homology 3 (BH3) domain of the human BAD, BIM, BIK, and Bcl-G genes in 60 advanced gastric adenocarcinomas, we used the polymerase chain reaction (PCR), single strand conformation polymorphism (SSCP), and DNA sequencing. RESULTS: The SSCP analysis revealed no mutations in the coding regions of the BH3 domain in the cancers. CONCLUSION: The data presented here indicate that proapoptotic Bcl-2 member genes, BAD, BIM, BIK, and Bcl-G, may not be mutated in human gastric carcinomas and suggest that these genes might be altered by mechanisms other mechanisms somatic mutation.
