Comparison of Batch Assay and Random Assay Using Automatic Dispenser in Radioimmunoassay.
- Author:
Seung Hwan MOON
1
;
Ho Young LEE
;
Sun Young SHIN
;
Gyeong Sun MIN
;
Hyun Joo LEE
;
Su Jin JANG
;
ji Yeon KANG
;
Dong Soo LEE
;
June Key CHUNG
;
Myung Chul LEE
Author Information
1. Department of Nuclear Medicine, Seoul Metropolitan Government Seoul National University Boramae Medical Center, Seoul, Korea. debobkr@gmail.com
- Publication Type:Original Article
- Keywords:
Radioimmunoassay;
batch assay;
random assay;
standard curve;
coefficient of variation;
intraclass correlation coefficient
- MeSH:
Carcinoembryonic Antigen;
Humans;
Prostate-Specific Antigen;
Radioimmunoassay;
Thyroxine;
Triiodothyronine
- From:Nuclear Medicine and Molecular Imaging
2009;43(4):323-329
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Radioimmunoassay (RIA) was usually performed by the batch assay. To improve the efficiency of RIA without increase of the cost and time, random assay could be a choice. We investigated the possibility of the random assay using automatic dispenser by assessing the agreement between batch assay and random assay. MATERIALS AND METHODS: The experiments were performed with four items; Triiodothyronine (T3), free thyroxine (fT4), Prostate specific antigen (PSA), Carcinoembryonic antigen (CEA). In each item, the sera of twenty patients, the standard, and the control samples were used. The measurements were done 4 times with 3 hour time intervals by random assay and batch assay. The coefficient of variation (CV) of the standard samples and patients' data in T3, fT4, PSA, and CEA were assessed. ICC (Intraclass correlation coefficient) and coefficient of correlation were measured to assessing the agreement between two methods. RESULTS: The CVs (%) of T3, fT4, PSA, and CEA measured by batch assay were 3.2+/-1.7%, 3.9+/-2.1%, 7.1+/-6.2%, 11.2+/-7.2%. The CVs by random assay were 2.1+/-1.7%, 4.8+/-3.1%, 3.6+/-4.8%, and 7.4+/-6.2%. The ICC between the batch assay and random assay were 0.9968 (T3), 0.9973 (fT4), 0.9996 (PSA), and 0.9901 (CEA). The coefficient of correlation between the batch assay and random assay were 0.9924(T3), 0.9974 (fT4), 0.9994 (PSA), and 0.9989 (CEA) (p<0.05). CONCLUSION: The results of random assay showed strong agreement with the batch assay in a day. These results suggest that random assay using automatic dispenser could be used in radioimmunoassay.
