Comparison of Amicus and CS-3000 Plus for the Collection of Peripheral Blood Stem Cells.
- Author:
Jin Sol LEE
1
;
Duck CHO
;
Myung Geun SHIN
;
Dong Wook RYANG
;
Je Jung LEE
;
Hyeoung Joon KIM
;
Hong Jae CHAE
;
Jong Hee SHIN
;
Soon Pal SUH
Author Information
1. Department of Laboratory Medicine, Chonnam National University Medical School, Gwangju, Korea. dwryang@chonnam.ac.kr
- Publication Type:Original Article
- Keywords:
Peripheral blood stem cell collection;
Amicus;
CS-3000 plus;
Platelet contamination
- MeSH:
Autografts;
Blood Cells;
Blood Component Removal;
Cell Count;
Delivery of Health Care;
Granulocyte-Macrophage Progenitor Cells;
Hand;
Hematopoietic Stem Cells;
Humans;
Korea;
Platelet Count;
Platelet Transfusion;
Retrospective Studies;
Stem Cells*;
Thrombocytopenia;
Tissue Donors
- From:Korean Journal of Blood Transfusion
2006;17(1):39-47
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: The efficient collection of peripheral blood stem cells (PBSC) from donors who donate for allogeneic transplants as well as from patients undergoing autologous transplants is essential for a successful transplant. Recently, the Amicus cell separator and the associated MNC collection computer software program for PBSC collection were introduced in Korea. METHODS: Two apheresis machines (Amicus, Baxter Healthcare; and CS-3000 plus, Baxter Healthcare) were compared retrospectively. A total number of 144 procedures were performed on 14 donors and 28 patients. The pre- and post-apheresis complete blood cell (CBC) counts and the number of hematopoietic progenitor cells (HPC) were determined in the peripheral blood from the subjects. The CBC, HPC, CD34+ cell counts and the level of colony-forming unit-granulocyte-macrophages (CFU-GM) were measured in the PBSC product collected from both machines. RESULTS: Both machines collected a similar number of CD34+ cells from the donors and patients. On the other hand, the Amicus collected significantly more nucleated cells, MNCs, HPCs and CFU-GM in the patients with significantly less RBC contamination than those with CS-3000 plus. The decrease in the peripheral blood platelet counts in the donors and patients was more prominent after apheresis using the CS-3000 plus (117.00+/-42.75 x 10(3)/microliter, 61.22+/-43.62 x 10(3)/microliter) than Amicus (26.04+/-18.68 x 10(3)/microliter, 22.15+/-28.66 x 10(3)/microliter)(p<0.05). CONCLUSION: PBSC collection can be performed successfully using CS-3000 plus and Amicus. Amicus is superior to CS-3000 plus in avoiding apheresis-induced thrombocytopenia, and is expected to prevent unnecessary platelet transfusion.
