Detection of an AB(weak) Blood Group by Genotyping.
- Author:
Hye Yoon CHUNG
1
;
Mi Sook YOON
;
Dong Chan KIM
;
Young Jin KIM
;
Sung Sup PARK
;
Kyou Sup HAN
Author Information
1. Department of Laboratory Medicine, Seoul National University Hospital, Seoul, Korea. kshanmd@snu.ac.kr
- Publication Type:Case Report
- Keywords:
ABO blood group;
Genotyping;
PCR-RFLP
- MeSH:
Adsorption;
Alleles;
Blood Transfusion;
DNA;
Erythrocytes;
Exons;
Humans;
Male;
Saliva;
Transferases
- From:Korean Journal of Blood Transfusion
2006;17(1):71-76
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Since precise ABO blood group typing is the most important part of a safe blood transfusion, the cause of an ABO discrepancy should be determined before issuing blood. The authors confirmed the ABO blood group of a 25-year-healthy male using genotyping method in those who showed an ABO blood group discrepancy between the cell and serum types. ABO typing, saliva test, adsorption elution test and serum transferase assay were performed and the presence of a weak B substance and B transferase was suspected without any evidence of the B antigen on red blood cells. Polymerase-chain-reaction restriction-fragment-length-polymorphism (PCR-RFLP) analysis was performed using the restriction enzymes including BstE II, Pvu II, BssH II, Alu I, and Mva I. The genotyping result showed a normal A allele and B allele. DNA analysis of exons 6 and 7 revealed normal A and B sequences. Therefore, we confirmed A(1)B(weak) blood type.
