Effects of interleukin-10 on chemokine KC gene expression by mouse peritoneal macrophages in response to Candida albicans.
10.3346/jkms.1999.14.5.480
- Author:
Hee Sun KIM
1
;
Dong Hoon SHIN
;
Sung Kwang KIM
Author Information
1. Department of Microbiology, College of Medicine, Yeungnam University, Taegu, Korea. heesun@medical.yeungnam.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Chemokines;
Candida albicans;
Gene expression
- MeSH:
Animal;
Blotting, Northern;
Candida albicans/metabolism*;
Cells, Cultured;
Chemotactic Factors/genetics*;
Dactinomycin/pharmacology;
Dose-Response Relationship, Drug;
Gene Expression Regulation/drug effects*;
Growth Substances/genetics*;
Interleukin-10/pharmacology*;
Interleukin-10/metabolism;
Macrophages/physiology*;
Mice;
Mice, Inbred BALB C;
Nucleic Acid Synthesis Inhibitors/pharmacology;
RNA, Messenger/metabolism;
RNA, Messenger/drug effects
- From:Journal of Korean Medical Science
1999;14(5):480-486
- CountryRepublic of Korea
- Language:English
-
Abstract:
Chemokine KC has been considered to be a murine homologue of human GRO/MGSA and was identified as chemoattractant for monocytes and neutrophils. This study examined the expression of KC mRNA in thioglycollate-elicited mouse peritoneal macrophages that were stimulated in vitro with Candida albicans (CA). Also examined were the inhibitory effects of IL-10 on the CA-induced expression of KC gene by Northern blot analysis. CA was found to induce chemokine gene expression in a gene-specific manner, CXC chemokine IP-10 mRNA expression was not detected in CA-stimulated macrophages. Maximum KC mRNA expression was observed approximately 2 hr after adding CA. The inhibitory action of IL-10 to CA-induced KC mRNA expression on mouse peritoneal macrophages was independent on concentration and stimulation time of IL-10 and was observed approximately one hour after adding IL-10 and CA simultaneously. IL-10 produced a decrease in the stability of KC mRNA, and CA-stimulated macrophages with cycloheximide blocked the suppressive effect of IL-10. These results suggest that CA also induces chemokine KC from macrophages, and IL-10 acts to destabilize CA-induced KC mRNA and de novo synthesis of an intermediate protein is a part of the IL-10 suppressive mechanism.
