Detection of chromosome aberrations in interphase nuclei using fluorescence in situ hybridization technique.
10.3346/jkms.1993.8.4.257
- Author:
Hyung Geun SONG
1
;
Seon Ok CHOI
;
In Beom KANG
Author Information
1. Department of Pathology, Chungbuk National University College of Medicine, Korea.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Fluorescence in situ hybridization;
Interphase cytogenetics;
Chromosome aberration
- MeSH:
Adolescent;
Adult;
Cell Nucleus/*ultrastructure;
Child;
Chromosome Aberrations/*physiology;
Chromosome Banding;
Female;
Humans;
In Situ Hybridization, Fluorescence;
Infant, Newborn;
Interphase/*physiology;
Male
- From:Journal of Korean Medical Science
1993;8(4):257-261
- CountryRepublic of Korea
- Language:English
-
Abstract:
We report here several experiences of interphase cytogenetics, using fluorescence in situ hybridization (FISH) technique, for the detection of chromosome aberrations. FISH, using alpha satellite specific probes of 18, X, Y chromosomes, was done in interphase nuclei from peripheral blood of patients with Edwards' syndrome, Klinefelter's syndrome and Turner's syndrome with healthy male and female controls, respectively. The distributions of fluorescent signals in 100 interphase nuclei were well correlated with metaphase findings. Nowadays FISH plays an increasingly important role in a variety of research areas, including cytogenetics, prenatal diagnosis, tumor biology, gene amplification and gene mapping.
