Effect of Etomidate on the Expressions of c-JUN and Heat Shock Protein 70 Induced by Kainic Acid in Rats.
10.4097/kjae.2004.47.2.253
- Author:
Jong Hwan LEE
1
;
Yu Mi LEE
;
Byung Joon CHANG
;
So Jin PARK
;
Ji Yeon LEE
;
Joung Uk KIM
;
Seung Woo KU
Author Information
1. Department of Anatomy, College of Veterinary Medicine, University of KonKuk, Korea.
- Publication Type:Original Article
- Keywords:
c-JUN;
etomidate;
hippocampus;
heat shock protein 70;
kainic acid;
neurotoxicity
- MeSH:
Animals;
Blotting, Western;
Cell Death;
Etomidate*;
Heat-Shock Proteins*;
Hippocampus;
Hot Temperature*;
HSP70 Heat-Shock Proteins*;
Kainic Acid*;
Neurons;
Neuroprotective Agents;
Rats*;
Rosaniline Dyes
- From:Korean Journal of Anesthesiology
2004;47(2):253-259
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: The present investigation was undertaken to evaluate the neuroprotective effect of etomidate against kainic acid (KA) induced neurotoxicity in rats by Western immunoblotting for heat shock protein 70 (HSP-70), c-JUN, and by acid-fuchsin histochemistry. METHODS: Etomidate (20 mg/kg, i.p.) was administered in sequence, first being just one hour after KA (10 mg/kg, i.p.) injection, then three times one hour intervals. Neuronal damage in hippocampus was evaluated by using acid-fuchsin stain to detect cell death and HSP-70 and c-JUN induction as an index of cell injury at 3, 6, 24 and 48 h after the administration of KA. RESULTS: Acid fuchsin positive neurons were increased in the CA1 and CA3 regions of the hippocampus after KA injection, but were significantly decreased by etomidate injection (P <0.01). Etomidate administration also significantly suppressed the KA-induced induction of c-JUN and HSP-70 in both regions of the rat hippocampus. CONCLUSIONS: These results suggest that etomidate has a protective effect on hippocampal neurons against KA-induced neurotoxicity.
