Combined Application of Bicuculline and 4-Aminopyridine-Induced Epileptiform Activity are Resistant to Conventional Anticonvulsants and AMPA in Young Rat Visual Cortex.
- Author:
Byung Jun CHOI
1
Author Information
1. Department of Pediatrics, College of Medicine, The Catholic University of Korea, Seoul, Korea. choibj@catholic.ac.kr
- Publication Type:Original Article
- Keywords:
GABA;
Seizure;
Visual cortex
- MeSH:
4-Aminopyridine;
6-Cyano-7-nitroquinoxaline-2,3-dione;
alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid*;
Animals;
Anticonvulsants*;
Bicuculline*;
Brain;
Carbamazepine;
gamma-Aminobutyric Acid;
Rats*;
Rats, Sprague-Dawley;
Receptors, AMPA;
Seizures;
Synaptic Transmission;
Visual Cortex*
- From:Journal of Korean Epilepsy Society
2006;10(1):35-40
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: The goal of the present study was to find out whether blockade of GABAergic synaptic transmission by bicuculline (BIC) in the presence of 4-aminopyridine (4-AP) would lead to expression or suppression of epileptiform activity in the immature brain and to observe the effect of commonly used anticonvulsants (valproic acid (VPA), carbamazepine (CBZP)) and 6-cyano-7-nitroquinoxalinedione (CNQX) on that epileptiform activity. METHODS: The visual cortex slices were obtained from 14-18 day-old Sprague-Dawley rats. Extracellular cellular recording was performed to observe the induction of epileptiform activity perfused by artificial CSF (ACSF) with combined application of BIC and 4-AP and the effect of VPA, CBZP and CNQX on that epileptiform activity for at least 1 hour. RESULTS: Epileptiform activity perfused by ACSF with combined application of BIC and 4-AP was insensitive to commonly used anticonvulsants (VPA, CBZP) and sensitive to CNQX. CONCLUSION: This study suggests that the epileptiform activity induced by combined application of BIC and 4-AP is present even in immature visual cortex slices. And, the blockade of GABAergic inhibition by BIC under 4-AP showed the increase of immature brain excitability as mature brain. The attenuation of that epileptiform activity by a-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) antagonist (CNQX) showed that AMPA receptor had relevance to the induction of that epileptiform activity.
