Glutathione Protects Brain Endothelial Cells from Hydrogen Peroxide-Induced Oxidative Stress by Increasing Nrf2 Expression.
- Author:
Juhyun SONG
1
;
So Mang KANG
;
Won Taek LEE
;
Kyung Ah PARK
;
Kyoung Min LEE
;
Jong Eun LEE
Author Information
- Publication Type:Original Article
- Keywords: glutathione (GSH); murine brain endothelial cells (bEnd.3 cells); apoptosis; hydrogen peroxide (H2O2); Reactive oxygen species (ROS); nuclear factor erythroid 2-related factor 2 (Nrf2)
- MeSH: Alzheimer Disease; Apoptosis; Brain Injuries; Brain*; Endothelial Cells*; Glutathione*; Huntington Disease; Hydrogen Peroxide; Hydrogen*; Neurodegenerative Diseases; Nitric Oxide; Oxidative Stress*; Reactive Oxygen Species; Tight Junction Proteins
- From:Experimental Neurobiology 2014;23(1):93-103
- CountryRepublic of Korea
- Language:English
- Abstract: Glutathione (GSH) protects cells against oxidative stress by playing an antioxidant role. Protecting brain endothelial cells under oxidative stress is key to treating cerebrovascular diseases and neurodegenerative diseases including Alzheimer's disease and Huntington's disease. In present study, we investigated the protective effect of GSH on brain endothelial cells against hydrogen peroxide (H2O2). We showed that GSH attenuates H2O2-induced production of nitric oxide (NO), reactive oxygen species (ROS), and 8-Oxo-2'-deoxyguanosine (8-OHdG), an oxidized form of deoxiguanosine. GSH also prevents H2O2-induced reduction of tight junction proteins. Finally, GSH increases the level of nuclear factor erythroid 2-related factor 2 (Nrf2) and activates Nrf2-mediated signaling pathways. Thus, GSH is a promising target to protect brain endothelial cells in conditions of brain injury and disease.
