Static magnetic fields promote osteoblastic/cementoblastic differentiation in osteoblasts, cementoblasts, and periodontal ligament cells.
10.5051/jpis.2017.47.5.273
- Author:
Eun Cheol KIM
1
;
Jaesuh PARK
;
Il Keun KWON
;
Suk Won LEE
;
Su Jung PARK
;
Su Jin AHN
Author Information
1. Department of Oral and Maxillofacial Pathology, Institute of Oral Biology, Kyung Hee University School of Dentistry, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Bone regeneration;
Periodontal guided tissue regeneration;
Relative biological effectiveness;
Signal transduction;
Wnt proteins
- MeSH:
Alkaline Phosphatase;
Blotting, Western;
Bone Regeneration;
Calcium;
Dental Cementum*;
Dental Prosthesis;
Gene Expression;
Glycogen Synthase;
Guided Tissue Regeneration, Periodontal;
Humans;
Immunohistochemistry;
JNK Mitogen-Activated Protein Kinases;
Magnetic Fields*;
Miners;
Osteoblasts*;
Periodontal Diseases;
Periodontal Ligament*;
Phosphorylation;
Polymerase Chain Reaction;
Protein Kinases;
Regeneration;
Relative Biological Effectiveness;
Reverse Transcription;
RNA, Messenger;
Signal Transduction;
Wnt Proteins
- From:Journal of Periodontal & Implant Science
2017;47(5):273-291
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: Although static magnetic fields (SMFs) have been used in dental prostheses and osseointegrated implants, their biological effects on osteoblastic and cementoblastic differentiation in cells involved in periodontal regeneration remain unknown. This study was undertaken to investigate the effects of SMFs (15 mT) on the osteoblastic and cementoblastic differentiation of human osteoblasts, periodontal ligament cells (PDLCs), and cementoblasts, and to explore the possible mechanisms underlying these effects. METHODS: Differentiation was evaluated by measuring alkaline phosphatase (ALP) activity, mineralized nodule formation based on Alizarin red staining, calcium content, and the expression of marker mRNAs assessed by reverse transcription polymerase chain reaction (RT-PCR). Signaling pathways were analyzed by western blotting and immunocytochemistry. RESULTS: The activities of the early marker ALP and the late markers matrix mineralization and calcium content, as well as osteoblast- and cementoblast-specific gene expression in osteoblasts, PDLCs, and cementoblasts were enhanced. SMFs upregulated the expression of Wnt proteins, and increased the phosphorylation of glycogen synthase kinase-3β (GSK-3β) and total β-catenin protein expression. Furthermore, p38 and c-Jun N-terminal kinase (JNK) mitogen-activated protein kinase (MAPK), and nuclear factor-κB (NF-κB) pathways were activated. CONCLUSIONS: SMF treatment enhanced osteoblastic and/or cementoblastic differentiation in osteoblasts, cementoblasts, and PDLCs. These findings provide a molecular basis for the beneficial osteogenic and/or cementogenic effect of SMFs, which could have potential in stimulating bone or cementum formation during bone regeneration and in patients with periodontal disease.
