Autoproliferation of Fibroblast by Exposure to Crystalline Silica - Evaluation by H2O2 and PDGF-AA and TGF beta.
- Author:
Byoung Young AHN
1
;
Kyoung Ah KIM
;
Je Hyeok MUN
;
Jin Sook JEONG
;
Eun Kyung KIM
;
Young LIM
Author Information
1. Department of Occupational and Environmental Medicine, St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Korea. nglim@cmc.cuk.ac.kr
- Publication Type:Original Article
- Keywords:
Platelet-derived growth factor-AA;
Transforming growth factor beta;
Crystalline silica;
Rat fibroblast
- MeSH:
Animals;
Crystallins*;
Enzyme-Linked Immunosorbent Assay;
Fibroblasts*;
Gene Expression;
Intercellular Signaling Peptides and Proteins;
Neutral Red;
Rats;
Reactive Oxygen Species;
Silicon Dioxide*;
Transforming Growth Factor beta;
Transforming Growth Factors
- From:Korean Journal of Occupational and Environmental Medicine
2000;12(2):209-217
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVES: The aim of this study is to find out the activity of autoproliferation of ratfibroblast exposed to crystalline silica and the role of mediators secreted from rat fibroblast. METHODS: The effect of alpha-quartz on production of growth factor (platelet-derived growth factor-AA and transforming growth factor beta)from rat fibroblasts were evaluated by ELISA and immunocytochemical analysis. Gene expression of these growth factors in rat fibrobast exposed to crystalline silica was evaluated by RT-PCR. Furthermore, fibroblast proliferation by culture supernatant of rat fibroblast was assayed by the neutral red test. RESULTS: The amounts of H2O2 and growth factors synthesized in rat fibroblasts were significantly increased by the stimulation of crystalline silica(alpha-quartz), which showed the dose-dependent manner to the concentration of alpha-quartz with the maximum response at the dosage of 100 microgram/cm2. The result of RT-PCR demonstrated that alpha-quartz induced gene expression of PDGF-AA and TGFbeta in rat fibroblast. We also found that supernatant of alpha-quartz-cocultured rat fibroblast induced a significant proliferation of fibroblast. CONCLUSION: Crystalline silica directly induce functional change in fibroblast such as increased release of reactive oxygen species and growth factors. The products of these functional change promote fibroblast proliferation via autocrine loop.
