The effect of fentanyl and midazolam on in vitro fertilization and early development of mouse embryo.
- Author:
Sang Yoon JUNG
;
Eun Joo LEE
;
Hoe Saeng YANG
;
Jae Chul SIM
- Publication Type:In Vitro ; Original Article
- Keywords:
Anesthetics;
IVF;
Fentanyl;
Midazolam;
Mouse oocyte
- MeSH:
Anesthetics;
Animals;
Blastocyst;
Embryonic Development;
Embryonic Structures*;
Female;
Fentanyl*;
Fertilization;
Fertilization in Vitro*;
Mice*;
Midazolam*;
Oocytes;
Pregnancy
- From:Korean Journal of Obstetrics and Gynecology
2000;43(6):955-960
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
OBJECTIVE: To assess the effect of fentanyl and midazolam on in vitro fertilization rate and early embryo development in a mouse IVF model. METHODS: Mouse oocytes were exposed in vitro to fentanyl at a concentration of 0(control), 50, 250, 500, 1000, 5000 pg/ml, and midazolam, 0(control), 2.5, 12.5, 25, 50, 250 ng/ml for 30 minutes, washed and inseminated. Thereafter fertilization was assessed. And subsequent in vitro development to the blastocyst stage was monitored daily. RESULTS: Where fertilization occurred, subsequent embryo cleavage and development up to the blastocyst stage was affected significantly by the presence of fentanyl and midazolam solution in the medium(i.e., 14% to 31%, 10% to 35%), in comparison with control group( 60%, 62%). CONCLUSION: It can be concluded from these experiments that even a brief exposure of cumulus enclosed oocytes to a low concentration of fentanyl, midazolam is deleterious to subsequent cleavage.
