Membrane Trafficking of Collecting Duct Water Channel Protein AQP2 Regulated by Akt/AS160.
- Author:
Hyun Jun JUNG
1
;
Tae Hwan KWON
Author Information
- Publication Type:Review
- Keywords: aquaporin 2; protein kinase B; protein transport; rab GTP-binding proteins; TBC1D4 protein
- MeSH: Animals; Aquaporin 2; Cell Cycle; Cell Death; Cell Membrane; Deamino Arginine Vasopressin; Gene Silencing; Intercellular Signaling Peptides and Proteins; Kidney Tubules, Collecting; Membranes; Mice; Microscopy, Confocal; Phosphatidylinositol 3-Kinase; Phosphotransferases; Protein Transport; Proto-Oncogene Proteins c-akt; rab GTP-Binding Proteins; RNA, Small Interfering; Vasopressins; Water
- From:Electrolytes & Blood Pressure 2010;8(2):59-65
- CountryRepublic of Korea
- Language:English
- Abstract: Akt (protein kinase B (PKB)) is a serine/threonine kinase that acts in the phosphatidylinositol 3-kinase (PI3K)/Akt signaling pathway. The PI3K/Akt signaling pathway, triggered by growth factors and hormones including vasopressin, is an important pathway that is widely involved in cellular mechanisms regulating transcription, translation, cell growth and death, cell proliferation, migration, and cell cycles. In particular, Akt and Akt substrate protein of 160 kDa (AS160) are likely to participate in the trafficking of aquaporin-2 (AQP2) in the kidney collecting duct. In this study, we demonstrated that 1) small interfering RNA (siRNA)-mediated gene silencing of Akt1 significantly decreased Akt1 and phospho-AS160 protein expression; and 2) confocal laser scanning microscopy of AQP2 in mouse cortical collecting duct cells (M-1 cells) revealed AS160 knockdown by siRNA increased AQP2 expression in the plasma membrane compared with controls, despite the absence of dDAVP stimulation. Thus, the results suggest that PI3K/Akt pathways could play a role in AQP2 trafficking via the AS160 protein.
