Effect of Genistein on the Survival and Production of Nitric Oxide in Trabecular Meshwork Cells.
10.3341/jkos.2011.52.8.970
- Author:
Jung Heum HONG
1
;
Yun Young KIM
;
Jae Woo KIM
Author Information
1. Department of Ophthalmology, Catholic University of Daegu School of Medicine, Daegu, Korea. jwkim@cu.ac.kr
- Publication Type:Original Article
- Keywords:
Genistein;
Nitric oxide;
Survival;
Trabecular meshwork cells
- MeSH:
Genistein;
Humans;
Nitric Oxide;
Trabecular Meshwork
- From:Journal of the Korean Ophthalmological Society
2011;52(8):970-974
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: To investigate the effects of genistein on the survival and production of nitric oxide in cultured human trabecular meshwork cells (HTMC). METHODS: Primarily cultured HTMC were exposed to 0, 1, and 10 microm genistein using serum-deprived media. Production of nitric oxide and eNOS activity were assessed with the Griess assay and RT-PCR after exposure to genistein for 10 min and one day. Cellular survival was assessed via MTT assay after exposure to genistein for one day. RESULTS: Genistein significantly increased the production of nitric oxide after exposure for 1 min at 10 microm and for 1 day at 1 microm under serum-deprived conditions. Genistein increased eNOS activity and cellular survival in HTMC. CONCLUSIONS: Genistein increases cellular survival under serum-deprived conditions, accompanied with an increase in nitric oxide production after both short-term and long-term exposures.
