Effect of Ethanol on the Regulation of Smooth Muscle Tone in Rat Aorta.
10.4070/kcj.2001.31.2.230
- Author:
Duk Joon SUH
;
Hae Rahn BAE
;
Dong Keun JUNG
;
Joong Hee LEE
- Publication Type:Original Article
- Keywords:
ethanol;
butanol;
Protein Kinase C;
Phospholipase D
- MeSH:
1-Butanol;
Acetylcholine;
Animals;
Aorta*;
Ethanol*;
Isometric Contraction;
Muscle, Smooth*;
Phenylephrine;
Phorbol 12,13-Dibutyrate;
Phospholipase D;
Protein Kinase C;
Rats*;
Relaxation;
tert-Butyl Alcohol;
Vasoconstrictor Agents
- From:Korean Circulation Journal
2001;31(2):230-237
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND AND OBJECTIVES: The aim of this study was to determine the effect of ethanol on the regulation of vascular tone. MATERIAL AND METHODS: Using rat aorta ring, isometric contraction and 45Ca uptake were measured. Phorbol 12,13-dibutyrate (PDBu), phenylephrine, KCl were used for the regulation of smooth muscle tone. RESULTS: Ethanol induced transient contraction in rat aorta ring by dose-dependent manner. Ethanol suppressed the dose dependent contractile responses of vascular strip by phenylephrine, KCl and PDBu. Endothelium-dependent relaxation by acetylcholine was inhibited by ethanol. Ethanol depressed 45Ca uptake by high KCl but not by phenylephrine or PDBu in rat aorta. n-butanol selectively suppressed tonic contraction by high KCl, but t-butanol did not at the same concentration of butanol in rat aorta. PDBu-induced contraction was selectively suppressed by n-butanol but not by t-butanol. CONCLUSIONS: These findings suggest that the action of ethanol on phospholipase D is involved in the decreased response of rat aorta strip by vasoconstrictors.
