Prevalence of Complement-Mediated Cell Lysis-like Gene (sicG) in Streptococcus dysgalactiae subsp. equisimilis Isolates From Japan (2014–2016).
10.3343/alm.2017.37.4.297
- Author:
Takashi TAKAHASHI
1
;
Tomohiro FUJITA
;
Akiyoshi SHIBAYAMA
;
Yuzo TSUYUKI
;
Haruno YOSHIDA
Author Information
1. Laboratory of Infectious Diseases, Kitasato Institute for Life Sciences, Kitasato University, Toky, Japan. taka2si@lisci.kitasato-u.ac.jp
- Publication Type:Original Article
- Keywords:
Streptococcus dysgalactiae subsp. equisimilis;
Complement-mediated cell lysis-like gene;
Clonal complex;
emm genotype;
Skin and soft tissue infections;
Random amplified polymorphic DNA analysis;
Japan
- MeSH:
Animals;
DNA;
Humans;
Japan*;
Joints;
Multilocus Sequence Typing;
Pharynx;
Prevalence*;
Sputum;
Streptococcus*;
Suppuration
- From:Annals of Laboratory Medicine
2017;37(4):297-304
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Streptococcus dysgalactiae subsp. equisimilis (SDSE; a β-hemolytic streptococcus of human or animal origin) infections are emerging worldwide. We evaluated the clonal distribution of complement-mediated cell lysis-like gene (sicG) among SDSE isolates from three central prefectures of Japan. METHODS: Group G/C β-hemolytic streptococci were collected from three institutions from April 2014 to March 2016. Fifty-five strains (52 from humans and three from animals) were identified as SDSE on the basis of 16S rRNA sequencing data.; they were obtained from 25 sterile (blood, joint fluid, and cerebrospinal fluid) and 30 non-sterile (skin-, respiratory tract-, and genitourinary tract-origin) samples. emm genotyping, multilocus sequence typing, sicG amplification/sequencing, and random amplified polymorphic DNA (RAPD) analysis of sicG-positive strains were performed. RESULTS: sicG was detected in 30.9% of the isolates (16 human and one canine) and the genes from the 16 human samples (blood, 10; open pus, 3; sputum, 2; throat swab, 1) and one canine sample (open pus) showed the same sequence pattern. All sicG-harboring isolates belonged to clonal complex (CC) 17, and the most prevalent emm type was stG6792 (82.4%). There was a significant association between sicG presence and the development of skin/soft tissue infections. CC17 isolates with sicG could be divided into three subtypes by RAPD analysis. CONCLUSIONS: CC17 SDSE harboring sicG might have spread into three closely-related prefectures in central Japan during 2014–2016. Clonal analysis of isolates from other areas might be needed to monitor potentially virulent strains in humans and animals.
