Proteomic analysis of Helicobacter pylori J99 Outer Membrane Protein by Tandem Mass Spectrometry.
- Author:
Kyung Mi KIM
1
;
Seung Gyu LEE
;
Jung Soo JOO
;
Young Chul KWON
;
Dong Won BEA
;
Jea Young SONG
;
Hyung Lyun KANG
;
Woo Kon LEE
;
Myung Je CHO
;
Kwang Ho RHEE
;
Hee Shang YOUN
;
Seung Chul BAIK
Author Information
- Publication Type:Original Article
- Keywords: Helicobacter pylori; Outer membrane protein; Q-TOF MS
- MeSH: Electrophoresis; Helicobacter; Helicobacter pylori; Mass Spectrometry; Membrane Proteins; Membranes; Proteins; Proteome; Proton-Motive Force; Tandem Mass Spectrometry
- From:Journal of Bacteriology and Virology 2008;38(2):53-60
- CountryRepublic of Korea
- Language:English
- Abstract: The protein identity of sarcosine-insoluble outer membrane proteins (OMPs) of Helicobacter pylori J99 was determined with the basic study of understanding the function of proteins. A sarcosine-insoluble OMPs was resolved by two-dimensional electrophoresis with immobilized pH gradient strips. The most abundant proteins were shown in the alkaline pI regions (6.0~11.0) with molecular masses of 10 to 100 kDa. We have performed an extensive proteome analysis by quadrupole time of flight (Q-TOF) mass spectrometry (MS). Here, of 50 spots processed, 42 spots were identified, which represented 16 genes and we newly detected 8 kinds of proteins (JHP0119, JHP0388, JHP1046, JHP1405, JHP0073, JHP0551, JHP1382, JHP0552) from the sarcosin-insoluble fraction of H. pylori J99. Those may be used to elucidate the characterization of the OMPs of H. pylori J99, which will help identify new potential target proteins for vaccine development and drug therapy.
