Integrated Cell Culture-PCR Detection of Enteroviruses and Reoviruses in Water Sources in Gyeonggi-do.
- Author:
Kyung A KIM
1
;
Jong Chan KIM
;
Hoan Uck KO
;
Jung Bock LEE
;
Young Sug KIM
;
Yong Bae PARK
;
Myung Jin LEE
;
Myung Gill KIM
;
Jae Kwan KIM
;
Eun Mi PARK
Author Information
- Publication Type:Original Article
- Keywords: Integrated cell culture-PCR (ICC-PCR); Total culturable virus assay (TCVA); Enteroviruses; Reoviruses
- MeSH: Enterovirus; Viruses; Water
- From:Journal of Bacteriology and Virology 2008;38(2):77-87
- CountryRepublic of Korea
- Language:Korean
- Abstract: The integrated cell culture-PCR (ICC-PCR) method has been suggested as an improved method for detection of viruses in water environments. We tested 57 source waters including finished water samples in Gyeonggi-do for enteric viral contamination using total culturable virus assay (TCVA) using BGMK cells and ICC-PCR. Nineteen of the 57 source water samples (33.3%) exhibited the cytopathic effect (CPE) on BGMK cells and no finished water did exhibited CPE. Nineteen samples (33.3%) of the 57 were positive for reoviruses. For the enteroviruses, only 3 samples (5.3%) of the 57 samples showed positive results. By using ICC-PCR method, 202 flasks from source water samples were positive for enteroviruses and reoviruses. Three samples from source water were positive for both viruses. However, any flasks tested was not co-infected with two types of viruses. While the enteric viral frequencies in TCVA and ICC-PCR were similar, the viral frequency for reoviruses at first passage in two type of method was higher in ICC-PCR (94.7%) than TCVA (56.9%).
