Analysis of gene expression during odontogenic differentiation of cultured human dental pulp cells.
10.5395/rde.2012.37.3.142
- Author:
Min Seock SEO
1
;
Kyung Gyun HWANG
;
Hyongbum KIM
;
Seung Ho BAEK
Author Information
1. Department of Dentistry, Hanyang University College of Medicine, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Differentiation;
Gene set enrichment analysis;
Human dental pulp cell;
Microarray
- MeSH:
Apoptosis;
Dental Pulp;
Gene Expression;
Genes, vif;
Humans;
Microarray Analysis;
Odontoblasts;
Oligonucleotide Array Sequence Analysis;
Polymerase Chain Reaction;
RNA
- From:Restorative Dentistry & Endodontics
2012;37(3):142-148
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVES: We analyzed gene-expression profiles after 14 day odontogenic induction of human dental pulp cells (DPCs) using a DNA microarray and sought candidate genes possibly associated with mineralization. MATERIALS AND METHODS: Induced human dental pulp cells were obtained by culturing DPCs in odontogenic induction medium (OM) for 14 day. Cells exposed to normal culture medium were used as controls. Total RNA was extracted from cells and analyzed by microarray analysis and the key results were confirmed selectively by reverse-transcriptase polymerase chain reaction (RT-PCR). We also performed a gene set enrichment analysis (GSEA) of the microarray data. RESULTS: Six hundred and five genes among the 47,320 probes on the BeadChip differed by a factor of more than two-fold in the induced cells. Of these, 217 genes were upregulated, and 388 were down-regulated. GSEA revealed that in the induced cells, genes implicated in Apoptosis and Signaling by wingless MMTV integration (Wnt) were significantly upregulated. CONCLUSIONS: Genes implicated in Apoptosis and Signaling by Wnt are highly connected to the differentiation of dental pulp cells into odontoblast.
