Post-mortem re-cloning of a transgenic red fluorescent protein dog.
10.4142/jvs.2011.12.4.405
- Author:
So Gun HONG
1
;
Ok Jae KOO
;
Hyun Ju OH
;
Jung Eun PARK
;
Minjung KIM
;
Geon A KIM
;
Eun Jung PARK
;
Goo JANG
;
Byeong Chun LEE
Author Information
1. Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea. bclee@snu.ac.kr
- Publication Type:Brief Communication ; Research Support, Non-U.S. Gov't
- Keywords:
re-cloned dog;
RFP dog;
serial cloning;
somatic cell nuclear transfer;
transgenic dog
- MeSH:
Animals;
Animals, Genetically Modified;
Cloning, Organism/methods/*veterinary;
Dogs/*genetics;
Female;
Gastrointestinal Tract/metabolism;
Gene Expression Regulation;
Kidney/metabolism;
Liver/metabolism;
Luminescent Proteins/*genetics/metabolism;
Lung/metabolism;
Male;
Myocardium/metabolism;
Nuclear Transfer Techniques/veterinary;
Spleen/metabolism;
Trachea/metabolism
- From:Journal of Veterinary Science
2011;12(4):405-407
- CountryRepublic of Korea
- Language:English
-
Abstract:
Recently, the world's first transgenic dogs were produced by somatic cell nuclear transfer. However, cellular senescence is a major limiting factor for producing more advanced transgenic dogs. To overcome this obstacle, we rejuvenated transgenic cells using a re-cloning technique. Fibroblasts from post-mortem red fluorescent protein (RFP) dog were reconstructed with in vivo matured oocytes and transferred into 10 surrogate dogs. One puppy was produced and confirmed as a re-cloned dog. Although the puppy was lost during birth, we successfully established a rejuvenated fibroblast cell line from this animal. The cell line was found to stably express RFP and is ready for additional genetic modification.
