Peripheral Blood Stem Cell Collection with an Automated Blood Cell Separator.
- Author:
Byoung Kook KIM
;
Seon Yang PARK
;
Kyou Sup HAN
- Publication Type:Original Article
- MeSH:
Autografts;
Blood Cells*;
Humans;
Korea;
Stem Cells*;
Transplantation, Autologous
- From:Korean Journal of Blood Transfusion
1994;5(1):1-8
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Peripheral blood stem cells(PBSC) were collected for autologous transplantation for the first time in Korea wish Fenwal CS-3000 Plus blood cell separator. Twenty-nine procedures were performed for 7 patients without any incidents. GM-CSF(400ug) was administered daily for 5 days before PBSC collection and the collections were performed 3-6 successive days. A total of 3.5-11.4 x 109 mononuclear cells(MNCs) were collected from 7,000mL blood processed per procedure. The number of MNCs collected was mainly dependent on the concentration of MNCs in patient's blood(r = 0.68, p<0.01). We concluded that the PBSC trans plantation program could be set up without difficulty in Korea since the Fenwal CS-3000 Plus blood cell separator is already available in many institutions and the procedure used in this study is easy to follow.
