A Case of Neonatal Altoimmune Thrombocytopenia due to Anti-HLA-B7 +1=160 +B61.
- Author:
Kyou Sup HAN
;
Tae Hyun UM
;
Myoung Hee PARK
;
Yong Won PARK
;
Bo Moon SHIN
;
Sang Woo KIM
- Publication Type:Case Report
- MeSH:
Antibodies, Monoclonal;
Blood Platelets;
Chloroquine;
Enzyme-Linked Immunosorbent Assay;
Fathers;
Fluorescent Antibody Technique;
Hemagglutination;
Hematoma;
Hemorrhage;
Humans;
Infant, Newborn;
Mothers;
Parturition;
Platelet Count;
Platelet Membrane Glycoproteins;
Prothrombin Time;
Purpura;
Sensitivity and Specificity;
Thrombocytopenia*;
Tissue Donors;
Volunteers
- From:Korean Journal of Blood Transfusion
1994;5(1):45-51
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
We encountered a case of neonatal altoimmune thrombocytopenia(NAIT) due to anti-HLA-B7+B60+B61. Bilateral cephal hematoma and umbilical hematoma were noted at the time of birth. Purpura developed at the third day. Platelet count was 110,000 at birth and decreased to 66,000/micro liter at the day 4. Prothrombin time and partial prothrombin time were within normal limit. The mother's platelet count was 220,000/micro liter and she had no history of abnormal bleeding. Platelet antibody tests empolying mixed passive hemagglutination and immunofluorescence revealed that the mother's serum was reactive against the platelets from the father and the neonate, but was not reactive with her own platelets. Platelets from eight volunteer group 0 donors were tested with the mother's serum; seven were reactive and one was negative. The positive reactions were lost after chloroquine treatment of platelets. Antigen capture ELISA(ACE) and modified antigen capture ELISA employing monoclonal antibodies against platelet glycoproteins In, IIa, IIb, and IIIa were negative. Mother's serum was tested for lymphocytotoxicity against 49 donor ]ymphocytes and the specificity was found to be anti-HLA-B7+B60+B61. At the 9th day, one unit of platelet concentrate from the mother was transfused and the platelet count of the neonate rose up to 340,000/micro liter. The neonate was discharged at the day of sixteenth and the platelet count remained high thereafter.