Detection of a Familial Y/l5 Translocation by FISH, G-Banding, C-Banding, and Ag-NOR Stain.
- Author:
Kyung Mee LEE
;
Kyeong Hee KIM
;
Jin Yeong HAN
;
Goo Hwa JE
;
Lisa G SHAFFER
- Publication Type:Original Article
- MeSH:
Arm;
Centromere;
Chromosomes, Human, Pair 15;
DNA Probes;
Fetus;
Fluorescence;
Heterochromatin;
Humans;
In Situ Hybridization;
Male;
Mothers;
Pregnancy
- From:Korean Journal of Clinical Pathology
1997;17(6):1176-1181
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Prenatal chromosome analysis of amniotic cells at 18 weeks of gestation showed a male fetus to carry a large 15p+ derivative chromosome inherited from his mother. Extra genetic material on the short arm of chromosome IS was silver-negative with Ag-NOR (nucleolus organizer regions) stain, but stained darkly with C-banding method like the distal heterochromatic segment of the Y long arm. Fluorescence in situ hybridization (FISH) using two DNA probes (DYZ1 and D15Zl) showed a red fluorescent signal on 15p+ In addition to a green chromosome 15 centromere signal, confirming 15p to be from the distal Yq heterochromatin.
