DNA ploidy patterns in gastric adenocarcinoma.
10.3346/jkms.2000.15.2.159
- Author:
Jung Young KIM
1
;
Hye Jae CHO
Author Information
1. Department of Diagnostic Pathology, Inje University Sanggye Paik Hospital, Seoul, Korea
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Ploidies;
Ki-67 Antigen;
Adenocarcinoma;
Stomach Neoplasms
- MeSH:
Adenocarcinoma/pathology*;
Adenocarcinoma/genetics*;
Adenocarcinoma/chemistry;
Aged;
Aneuploidy*;
Cell Differentiation;
Cell Division;
Female;
Flow Cytometry;
Gastric Mucosa/pathology;
Human;
Ki-67 Antigen/analysis;
Male;
Middle Age;
Stomach Neoplasms/pathology*;
Stomach Neoplasms/genetics*;
Stomach Neoplasms/chemistry
- From:Journal of Korean Medical Science
2000;15(2):159-166
- CountryRepublic of Korea
- Language:English
-
Abstract:
To assess the value of DNA ploidy, flow cytometric analysis was performed on unfixed fresh materials obtained from 86 patients with gastric cancer who underwent stomach resection. We evaluated the DNA content of gastric carcinoma cells from four different sites and compared it with Ki-67 proliferating activity, and other pathologic parameters. The incidence of aneuploid and diploid was similar (48.8% vs. 51.1%). Early gastric carcinoma showed a higher rate of the diploid pattern (75%) compared to that of advanced gastric carcinoma 7.3%). DNA diploidy was noted increasingly in diffuse-type tumors according to uren, in signet ring cell type tumor according to WHO classification and in orly differentiated tumors (p<0.05). Well and moderately differentiated rcinomas revealed the aneuploid pattern more frequently than poorly fferentiated tumors. The aneuploidy was associated with high S phase fraction d high proliferative index. Aneuploidy was noted in the mucosa adjacent to the mor (26%), in the close normal-looking mucosa (7%) and in the remote rmal-looking mucosa (3%). This result suggest the possible role of field ncerization in the development of gastric adenocarcinoma.
