Fetal D positive blood detection by PCR in D negative preganant.
- Author:
Chae Seung LIM
1
;
Kap No LEE
;
Yong Kyun PARK
Author Information
1. Department of Clinical Pathology, College of Medicine, Korea University, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Rho(D);
Polymerase Chain Reaction;
Fetomaternal hemorrhage
- MeSH:
Female;
Fetomaternal Transfusion;
Fetus;
Gestational Age;
Polymerase Chain Reaction*;
Postpartum Period;
Pregnancy
- From:Korean Journal of Blood Transfusion
1997;8(2):97-101
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Presence of fetal D positive RBCs in maternal peripheral blood of D negative pregnants induce Rh alloimmunization. Early detection of fetal D positive RBCs would eliminate the risk to an D positive fetus in pregnancy. We demonstrate and evaluate relibility of a sensitive polymerase chain reaction(PCR)-based assay for the RHD fetus gene detection from maternal peripheral blood samples. METHODS: The amplification of RHD gene and RHCcEe gene site were done in peripheral blood sample(n=17) of nine D negative pregnants by polymerase chain reaction and evaluated the sensitivity of PCR genotyping in serially diluted D positive sample. If RHD amplicated band(189 bp) were found with RHCcEe band(136 bp) in peripheral blood of D negative pregnants, the results were interpreted as positive for fetomaternal hemorrhage detection. RESULTS: Gestational age distribution of samples were from 7 to 39 weeks include postpartum one day. The RHD typing by PCR showed good sensitivity to detect up to 0.05% fetomaternal hemorrhage. Two cases were positive by PCR among 17 samples and their gestaional age was 22 and 27 weeks. CONCLUSIONS: RHD PCR showed good sensitivity for fetomaternal hemorrhage detection and prophylaxis of Rho(D) alloimmunization should be carefully done by this results because fetomaternal hemorrhage were detected in early gestational age by PCR.
