Erythropoietin does not affect nitric oxide system in rats with chronic renal failure.
10.3346/jkms.2000.15.2.183
- Author:
Soo Wan KIM
1
;
Jong Un LEE
;
Dae Gill KANG
;
Kwon JUNG
;
Nam Ho KIM
;
Soon Pal SUH
;
Ki Chul CHOI
;
Young Joon KANG
Author Information
1. Department of Internal Medicine, Chonnam National University Medical School, Kwangju, Korea.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Renal Failure, Chronic;
Erythropoietin;
Hypertension;
Nitric Oxide
- MeSH:
Acetylcholine/pharmacology;
Anemia/metabolism;
Anemia/etiology;
Anemia/drug therapy*;
Animal;
Aorta, Thoracic/physiology;
Body Weight;
Erythropoietin/pharmacology*;
Hypertension, Renal/metabolism;
Hypertension, Renal/drug therapy;
Isometric Contraction/drug effects;
Kidney/enzymology;
Kidney Failure, Chronic/metabolism*;
Kidney Failure, Chronic/complications;
Male;
Nitrates/urine;
Nitrates/blood;
Nitric Oxide/metabolism*;
Nitric-Oxide Synthase/metabolism;
Nitrites/urine;
Nitrites/blood;
Nitroprusside/pharmacology;
Rats;
Rats, Sprague-Dawley;
Vasoconstriction/drug effects;
Vasoconstrictor Agents/pharmacology;
Vasodilator Agents/pharmacology
- From:Journal of Korean Medical Science
2000;15(2):183-188
- CountryRepublic of Korea
- Language:English
-
Abstract:
We investigated to see whether an altered role of nitric oxide (NO) system is involved in erythropoietin (EPO)-induced hypertension in chronic renal failure (CRF). Male Sprague-Dawley rats were five-sixths nephrectomized to induce CRF. Six weeks after the operation, EPO or vehicle was injected for another 6 weeks. Plasma and urine nitrite/nitrate (NOx) levels were determined. Expression of NO synthase (NOS) proteins in the aortae and kidneys were also determined. In addition, the isometric tension of isolated aorta in response to acetylcholine and nitroprusside was examined. Blood pressure progressively rose in CRF groups, the degree of which was augmented by EPO treatment. Plasma NOx levels did not differ among the groups, while urine NOx levels were lower in CRF groups. Endothelial NOS expression was lower in the kidney and aorta in CRF rats, which was not further affected by EPO-treatment. The inducible NOS expression in the kidney and aorta was not different among the groups. Acetylcholine and sodium nitroprusside caused dose-dependent relaxations of aortic rings, the degree of which was not altered by EPO-treatment. Taken together, EPO-treatment aggravates hypertension in CRF, but altered role of NO system may not be involved.
