Comparison of the Novel Human Papillomavirus 4 Auto-capillary Electrophoresis Test with the Hybrid Capture 2 Assay and with the PCR HPV Typing Set Test in the Detection of High-Risk HPV Including HPV 16 and 18 Genotypes in Cervical Specimens.
10.3346/jkms.2009.24.4.579
- Author:
Jin Hwa HONG
1
;
Seung Hun SONG
;
Jong Kee KIM
;
Jeong Hyun HAN
;
Jae Kwan LEE
Author Information
1. Department of Obstetrics and Gynecology, Guro Hospital, College of Medicine, Korea University, Seoul, Korea. jklee38@korea.ac.kr
- Publication Type:Original Article ; Comparative Study
- Keywords:
HPV Typing Methods;
ACE;
HC2;
HPV Typing Set Test
- MeSH:
Cervix Uteri/*virology;
DNA, Viral/analysis;
Electrophoresis, Capillary/*methods;
Female;
Gammapapillomavirus/genetics/*isolation & purification;
Genotype;
Human papillomavirus 16/genetics/*isolation & purification;
Human papillomavirus 18/genetics/*isolation & purification;
Humans;
Nucleic Acid Hybridization;
Polymerase Chain Reaction/methods;
Reagent Kits, Diagnostic;
Vaginal Smears
- From:Journal of Korean Medical Science
2009;24(4):579-584
- CountryRepublic of Korea
- Language:English
-
Abstract:
The aim of this study was to compare the novel human papillomavirus (HPV) detection method, the HPV 4 Auto-capillary Electrophoresis (ACE) test with the hybrid capture (HC) 2 assay for the detection of high-risk HPVs. In addition, we compared the HPV 4 ACE test with the polymerase chain reaction HPV Typing Set test for the detection of HPV 16 and HPV 18 genotypes. One hundred ninety-nine cervical swab samples obtained from women with previous abnormal Pap smears were subjected to testing with the three HPV tests. The HPV 4 ACE test and the HC 2 assay showed substantial agreement for detection of high-risk HPVs (85.4%, kappa=0.71). The HPV 4 ACE test also showed substantial agreement with the PCR HPV Typing Set test in the detection of HPV 16 and HP V 18 genotypes (89.9%, kappa=0.65). In correlation with cytologic results, the sensitivities and specificities of the HPV 4 ACE test and HC 2 assay were 92.9% vs. 92.9% and 48.1% vs. 50.8%, respectively, when high-grade squamous intraepithelial lesions were regarded as abnormal cytologies. The novel HPV 4 ACE test is a valuable tool for the detection of high-risk HPVs and for genotyping of HPV 16 and HPV 18.
