Apigenin Inhibits Tumor Necrosis Factor-alpha-Induced Production and Gene Expression of Mucin through Regulating Nuclear Factor-Kappa B Signaling Pathway in Airway Epithelial Cells.
10.4062/biomolther.2014.094
- Author:
Hyo Seok SEO
1
;
Mohamed Asaduzzaman SIKDER
;
Hyun Jae LEE
;
Jiho RYU
;
Choong Jae LEE
Author Information
1. Department of Pharmacology, School of Medicine, Chungnam National University, Daejeon 301-131, Republic of Korea. LCJ123@cnu.ac.kr
- Publication Type:Original Article
- Keywords:
Airway;
Mucin;
Apigenin
- MeSH:
Apigenin*;
Blotting, Western;
Down-Regulation;
Enzyme-Linked Immunosorbent Assay;
Epithelial Cells*;
Gene Expression*;
Mucins*;
Necrosis*;
NF-kappa B;
Phosphorylation;
Phosphotransferases;
Polymerase Chain Reaction;
Reverse Transcription;
Tumor Necrosis Factor-alpha
- From:Biomolecules & Therapeutics
2014;22(6):525-531
- CountryRepublic of Korea
- Language:English
-
Abstract:
In the present study, we investigated whether apigenin significantly affects tumor necrosis factor-alpha (TNF-alpha)-induced production and gene expression of MUC5AC mucin in airway epithelial cells. Confluent NCI-H292 cells were pretreated with apigenin for 30 min and then stimulated with TNF-alpha for 24 h or the indicated periods. The MUC5AC mucin gene expression and mucin protein production were measured by reverse transcription - polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA), respectively. Apigenin significantly inhibited MUC5AC mucin production and down-regulated MUC5AC gene expression induced by TNF-alpha in NCI-H292 cells. To elucidate the action mechanism of apigenin, effect of apigenin on TNF-alpha-induced nuclear factor kappa B (NF-kappaB) signaling pathway was also investigated by western blot analysis. Apigenin inhibited NF-kappaB activation induced by TNF-alpha. Inhibition of inhibitory kappa B kinase (IKK) by apigenin led to the suppression of inhibitory kappa B alpha (IkappaBalpha) phosphorylation and degradation, p65 nuclear translocation. This, in turn, led to the down-regulation of MUC5AC protein production in NCI-H292 cells. Apigenin also has an influence on upstream signaling of IKK because it inhibited the expression of adaptor protein, receptor interacting protein 1 (RIP1). These results suggest that apigenin can regulate the production and gene expression of mucin through regulating NF-kappaB signaling pathway in airway epithelial cells.
