Over-expression of MMP-3 in the fissured tissue of cleft lip and palate.
- Author:
Young Wook PARK
1
;
Bong Gi MIN
;
Ji Hyuck KIM
;
Soung Min KIM
;
Young Joon LEE
;
Sang Shin LEE
;
Suk Keun LEE
;
Huck Soo MOON
;
Je Geun CHI
Author Information
1. Department of Oral and Maxillofacial Surgery, College of Dentistry, Kangnung National University, Korea.
- Publication Type:Original Article
- Keywords:
Human;
Fetal;
Adult;
Cleft lip and palate;
Sebaceous gland;
MMP-3
- MeSH:
Adult;
Antibodies;
Cadherins;
Cathepsin G;
Cleft Lip*;
Follicular Cyst;
Humans;
Hyperplasia;
Lip;
Melanocytes;
Mouth Mucosa;
Outcome Assessment (Health Care);
Palate*;
Proliferating Cell Nuclear Antigen;
Sebaceous Glands;
Skin;
Vascular Endothelial Growth Factor A
- From:Journal of the Korean Association of Oral and Maxillofacial Surgeons
2006;32(1):19-26
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVE: In order to elucidate the retrogressive degeneration of orofacial cleft, the fissured tissues of prenatal and postnatal cleft lip and palate were examined by histological and immunohistochemical methods. DESIGN: Totally 42 cases of prenatal (n=17) and postnatal (n=25) cleft lip and/or palate were examined in comparison with 10 cases of normal lip and oral mucosa using immunohistochemical stainings of MMP-3, MMP-9, MMP-10, cathepsin G, PCNA, E-cadherin, TGase 2, HSP-70, vWF, and VEGF. MAIN OUTCOME MEASURES: In the fissured tissue the sebaceous glands were strongly positive for PCNA and grew into the underlying fibromuscular tissue (24/42). Some hyperplastic sebaceous glands of prenatal cleft lip produced infundibular follicular cyst (9/17). The skin and mucosal epithelia from the postnatal cleft lip and palate (10/25) showed severe basal hyperplasia (11/25) and melanocyte infiltration (7/25). RESULTS: The immunostaining of MMP-3 and HSP-70 were strongly positive in the hyperplastic sebaceous glands and nearby atrophying muscle bundles of the fissured tissue, while MMP-9, MMP-10, and cathepsin G were almost negative. The immunoreactions of the other antibodies used in this study were similar between in the fissured tissues and in the normal controls. CONCLUSIONS: These data suggest that the over-expression of MMP-3 is closely related to the sebaceous gland hyperplasia, epithelial dysplasia, and the muscle degeneration, and that the over-expression of MMP-3 in the fissured tissue may continuously aggravate the cleft condition in the later life.
