Bone Regeneration with MMP sensitive hyaluronic acid-based hydrogel, rhBMP-2 and nanoparticles in rat calvarial critical size defect(CSD) model.
- Author:
Jong Chul PARK
1
;
Sang Bae YU
;
Yong Il CHUNG
;
Gi Yoong TAE
;
Jung Ju KIM
;
Yong Doo PARK
;
Jeong Won JAHNG
;
Jong Ho LEE
Author Information
1. Department of Oral and Maxillofacial Surgery, School of Dentistry, Seoul National University, Korea. leejongh@snu.ac.kr
- Publication Type:Original Article
- Keywords:
matrix metalloproteinase(MMP);
hyaluronic acid;
controlled release;
rhBMP-2;
nanoparticle;
MMP sensitive hydrogel
- MeSH:
Animals;
Bone Marrow;
Bone Regeneration;
Calcium;
Hyaluronic Acid;
Hydrogel;
Nanoparticles;
Osteogenesis;
Rats;
Transplants
- From:Journal of the Korean Association of Oral and Maxillofacial Surgeons
2009;35(3):137-145
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
As an efficient controlled release system for rhBMP-2, a functional nanoparticle-hydrogel complex, incorporated with matrix metalloproteinase( MMP) sensitive peptide cross-linker, was developed and used as a bone transplant. In vivo bone formation was evaluated by soft x-ray, histology, alkaline phosphatase(ALP) activity and mineral contents analysis, based on the rat calvarial critical size defect(8mm in diameter) model.Significantly, effective bone regeneration was achieved with the rhBMP-2 loaded MMP sensitive hyaluronic acid(HA) based hydrogel- Nanoparticles(NP) complex, as compared to only MMP HA, the MMP HA-NP without rhBMP-2, or even with the rhBMP-2. These improvements included the formation pattern of bone and functional marrow, the degree of calcium quantification, and the ALP activity. These results indicate that the MMP sensitive HA with nano-particle complex can be a promising candidate for a new bone defect replacement matrix, and an enhanced rhBMP-2 scaffold.
