Gene Cloning of the Epstein-Barr Virus (EBV) Antigen Reactive with the Serum from EBV-infected Patients.
- Author:
Eung Soo HWANG
1
;
Jinhee KIM
;
Chung Gyu PARK
;
Yoon Hoh KOOK
;
Myung Sik CHOI
;
Ik Sang KIM
;
Sung Bae CHOI
;
Chang Yong CHA
Author Information
1. Department of Microbiology, Seoul National University College of Medicine, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Epstein-Barr virus;
Gene cloning;
Phage library;
Protein expression
- MeSH:
Antibody Formation;
Base Sequence;
beta-Galactosidase;
Burkitt Lymphoma;
Clone Cells*;
Cloning, Organism*;
DNA, Complementary;
Epstein-Barr Virus Infections;
Herpesviridae;
Herpesvirus 4, Human*;
Humans;
Immunoglobulin G;
Infectious Mononucleosis;
Recombinant Proteins;
Sequence Homology;
Serologic Tests
- From:Korean Journal of Infectious Diseases
2000;32(4):287-293
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Epstein-Barr virus (EBV) is causative agent of infectious mononucleosis and nasopharyngeal carcinoma and associated with Burkitt lymphoma and other tumors. The recombinant protein is needed for the rapid and sensitive serodiagnosis of EBV infection. METHODS: EBV gene encoding the protein reactive with the sera of EBV-infected patient was cloned and characterized with lambda gt11 expression library of cDNA of EBV B95-8 strain. RESULTS: The recombinant proteins from clone 12, 15 and 21 were expressed as 120, 118, 160 kDa-usion protein with beta-galactosidase, respectively, which were reactive with IgG anti-EBV antibody-positive sera, but not with anti-EBV antibody-negative sera. A homology search of the DNA sequence of the cloned gene with EBV B95-8 sequences revealed that those were located at 61716~62087, 61898~62085, and 102128~103158, respectively. These positions correspond to BFRF3, BFRF3, and BZLF1, respectively, which were reported as immunogenic protein which evoked strong and longstanding antibody response and had no sequence homology with the proteins of other herpesviruses. All the patients' sera were reactive with clone 12 protein, but only 5 out of 9 patients' sera were reactive with clone 21 protein. CONCLUSION: Clone 21 protein expressing BFRF3 fragment was immunoreactive in patient sera from natural EBV infection and was regarded as useful candidate for the serodiagnosis of EBV infection.
