High Glucose and Advanced Glycosylation Endproducts(AGE) Modulate the P-cadherin Expression in Glomerular Epithelial Cells(GEpC).
- Author:
Tae Sun HA
1
;
Hyun Hoe KOO
;
Hae Soo LEE
;
Ok Ja YOON
Author Information
1. Department of Pediatrics, Chungbuk National University, Cheongju, Chungbuk, Korea. tsha@chungbuk.ac.kr
- Publication Type:Original Article
- Keywords:
Diabetic nephropathy;
Advanced glycosylation endproducts;
P-cadherin;
Glomerular epithelial cells;
Podocyte
- MeSH:
Animals;
Blotting, Western;
Cadherins*;
Diabetic Nephropathies;
Filtration;
Glucose*;
Glycosylation*;
Podocytes;
Polymerase Chain Reaction;
Rats;
RNA, Messenger
- From:Journal of the Korean Society of Pediatric Nephrology
2005;9(2):119-127
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Podocytes are critical in maintaining the filtration barrier of the glomerulus and are dependent on the integrity of slit diaphragm(SD) proteins including nephrin, P-cadherin, and others. Diabetic proteinuric condition demonstrates defects in SD molecules as well as ultrastructural changes in podocytes. We examined the molecular basis for this alteration of SD molecules especially on P-cadherin as a candidate regulating the modulation of pathogenic changes in the barrier to protein filtration. METHODS: To investigate whether high glucose and AGE induce changes in SD, we cultured rat GEpC under normal(5 mM) or high glucose(30 mM) and AGE- or BSA-added conditions and measured the change of P-cadherin expression by Western blotting and RT- PCR. RESULTS: We found that administration of high glucose decreased the P-cadherin production significantly in the presence or absence of AGE by Western blotting. In RT-PCR high glucose with or without AGE also significantly decreased the expression of P-cadherin mRNA compared to those of controls. Such changes were not seen in the osmotic control. CONCLUSION: We suggest that high glucose with or without AGE suppresses the production of P-cadherin at the transcriptional level and that these changes may explain the functional changes of SD in diabetic conditions.
