Immunohistochemical Staining of Amyloid Deposit with Monoclonal Anti - keratin Antibodies in Primary Localized Cutaneous Amyloidosis.
- Author:
Kwang Hyun CHO
;
Seung Ho CHANG
;
Jeong Aee KIM
;
Yoo Shin LEE
;
Eui Keun HAM
- Publication Type:Original Article
- Keywords:
Amyloid P;
Cutaneous amyloidosis;
Keratin
- MeSH:
Amyloid*;
Amyloidosis*;
Antibodies*;
Catalytic Domain;
Dermis;
Immunoenzyme Techniques;
Lichens;
Paraffin;
Peptides;
Plaque, Amyloid*
- From:Korean Journal of Dermatology
1990;28(3):308-314
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
Nine cases of primary localized cutaneous amyloidosis were studied by immunoperoxidase technique (ABC method) employing anti-keratin antibodies. All specimens were examined using consecutive paraffin sections to confirm the correspondence between amyloid existing area and reactive sites. Anti-keratin antibody 34pE which recognize 68, 58, 56.5, 56kd keratin peptides reacted with amyloid deposits in both lichen amyloidosus and macular amyloidosis. However, anti-keratin antibodies 34pB4 and 35pH did not react with amyloids. In general, Dylon staining positive material, keratin reacted with 34pE and amyloid P showed similar distribution in serial sections, but did not show the same one. Several keratin bodies reacted with 34pE, which were not stained with Dylon staining or antiamyloid P were found in the dermis of one specimen. These results suggest that immunohistochemical staining with antikeratin antibody 34pE using formalin-fixed, paraffin-embedded sections appeared to be a useful method in studying the histogenesis of primary localized cutaneous arnyloidosis.
