Development of Streptococcus sanguinis-, Streptococcus parasanguinis-, and Streptococcus gordonii-PCR Primers Based on the Nucleotide Sequences of Species-specific DNA Probes Screened by Inverted Dot Blot Hybridization.
- Author:
Soon Nang PARK
1
;
Joong Ki KOOK
Author Information
1. Korean Collection for Oral Microbiology, Department of Oral Biochemistry, and Oral Biology Research Institute, School of Dentistry, Chosun University, 375 Seosuk-dong, Dong-gu, Gwangju 501-759, Korea. jkkook@chosun.ac.kr
- Publication Type:Original Article
- Keywords:
Streptococcus gordonii;
Streptococcus sanguinis;
Streptococcus parasanguinis;
species-specific PCR primer;
IDBH
- MeSH:
Base Sequence;
Chimera;
DNA;
DNA Probes;
Polymerase Chain Reaction;
Streptococcus;
Streptococcus gordonii
- From:International Journal of Oral Biology
2013;38(2):43-49
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
The objective of this study was to develop PCR primers that are specific for Streptococcus sanguinis, Streptococcus parasanguinis, and Streptococcus gordonii. We designed the S. sanguinis-, S. parasanguinis-, and S. gordonii-specific primers, Ssa21-F3/Ssa21-R2, Spa17-F/Spa17-R, and Sgo41-F1/Sgo41-R1 respectively, based on the nucleotide sequences of the Ssa21, Spa17, and Sgo41 DNA probes that were screened using inverted dot blot hybridization (IDBH). The species-specificity of these primers was assessed against 43 strains of mitis group streptococci, including clinical strains of S. sanguinis, S. parasanguinis, and S. gordonii. The resulting PCR data revealed that species-specific amplicons had been obtained from all strains of the target species tested, and that none of these amplicons occurred in any other strains from other species. These results suggest that the Ssa21-F3/Ssa21-R2, Spa17-F/Spa17-R, and Sgo41-F1/Sgo41-R1 primers may be useful in detecting S. sanguinis, S. parasanguinis, and S. gordonii at the species level, respectively.
