Expression of hOGG1 protein during differentiation of HL-60 cells.
- Author:
Yun Song LEE
1
;
Kyeong Hoon LEE
;
Myung Hee CHUNG
Author Information
1. Division of Pharmacology, Sungkyunkwan University School of Medicine Suwon 440-746, Korea. yslee@skku.edu
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
cell differentiation;
DNA;
glycosylation;
reactive oxygen species;
oxidative stress
- MeSH:
Blotting, Western;
*Cell Differentiation;
Culture Media, Serum-Free/pharmacology;
DNA Glycosylases/*metabolism;
Enzyme Activation;
*Gene Expression Regulation, Enzymologic/drug effects;
Granulocytes/cytology/drug effects/metabolism;
HL-60 Cells;
Human;
Monocytes/cytology/drug effects/metabolism
- From:Experimental & Molecular Medicine
2003;35(2):98-105
- CountryRepublic of Korea
- Language:English
-
Abstract:
Human 8-oxo-G-DNA glycosylase 1 (hOGG1) is a DNA glycosylase to cleave 8-oxo-7,8-dihydroguanine (8-oxo-G), a mutagenic DNA adduct formed by oxidant stresses. Here, we examined hOGG1 protein expression and repair activity to nick a DNA strand at the site of 8-oxo-G during differentiation of hematopoietic cells using HL-60 cells. Overall expression of hOGG1 protein was increased during granulocytic differentiation of HL-60 cells induced by DMSO and monocytic differentiation by vitamine D3. Greater level of hOGG1 protein was expressed in DMSO-treated cells. However, change in the DNA nicking activity was not in parallel with the change in hOGG1 protein expression, especially in PMA-treated cells. In PMA- treated cells, the level of hOGG1 protein was lowered, even though the DNA nicking activity was elevated, in a manner similar to the changes in serum- deprived HL-60 cells. These results indicate that hOGG1 expression change during differentiation of hematopoietic stem cells for adaptation to new environments. And the DNA cleaving activity may require additional factor(s) other than expressed hOGG1 protein, especially in apoptotic cell death.
