Antioxidant Effect of Captopril and Enalapril on Reactive Oxygen Species-Induced Endothelial Dysfunction in the Rabbit Abdominal Aorta.
10.5090/kjtcs.2013.46.1.14
- Author:
Ji Hoon KIM
1
;
Hyuck KIM
;
Young Hak KIM
;
Won Sang CHUNG
;
Jung Kook SUH
;
Sung Jin KIM
Author Information
1. Department of Medicine, Sicho Subcenter, Seocheon County Public Health Center, Korea.
- Publication Type:Original Article
- Keywords:
Reactive oxygen species;
Angiotensin-converting enzyme inhibitors;
Captopril;
Enalapril;
Vasodilation
- MeSH:
Acetylcholine;
Amitrole;
Angiotensin-Converting Enzyme Inhibitors;
Antioxidants;
Aorta, Abdominal;
Baths;
Captopril;
Cardiovascular Diseases;
Ditiocarb;
Electrolysis;
Enalapril;
Endothelium;
Estradiol;
Hydrogen Peroxide;
Nitric Oxide;
Norepinephrine;
Oxygen;
Reactive Oxygen Species;
Superoxide Dismutase;
Superoxides;
Vasodilation
- From:The Korean Journal of Thoracic and Cardiovascular Surgery
2013;46(1):14-21
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: Reactive oxygen species (ROS) are known to be related to cardiovascular diseases. Many studies have demonstrated that angiotensin-converting enzyme inhibitors have beneficial effects against ROS. We investigated the antioxidant effect of captopril and enalapril in nitric oxide mediated vascular endothelium-dependent relaxations. MATERIALS AND METHODS: Isolated rabbit abdominal aorta ring segments were exposed to ROS by electrolysis of the organ bath medium (Krebs-Henseleit solution) after pretreatment with various concentrations (range, 10-5 to 3x10-4 M) of captopril and enalapril. Before and after electrolysis, the endothelial function was measured by preconstricting the vessels with norepinephrine (10-6 M) followed by the cumulative addition of acetylcholine (range, 3x10-8 to 10-6 M). The relevance of the superoxide anion and hydrogen peroxide scavenging effect of captopril and enalapril was investigated using additional pretreatments of diethyldithiocarbamate (DETCA, 0.5 mM), an inhibitor of Cu/Zn superoxide dismutase, and 3-amino-1,2,4-triazole (3AT, 50 mM), an inhibitor of catalase. RESULTS: Both captopril and enalapril preserved vascular endothelium-dependent relaxation after exposure to ROS in a dose-dependent manner (p<0.0001). Pretreatment with DETCA attenuated the antioxidant effect of captopril and enalapril (p<0.0001), but pretreatment with 3AT did not have an effect. CONCLUSION: Both captopril and enalapril protect endothelium against ROS in a dose-dependent fashion in isolated rabbit abdominal aortas. This protective effect is related to superoxide anion scavenging.
