Effect of Single Growth Factor and Growth Factor Combinations on Differentiation of Neural Stem Cells.
10.3340/jkns.2008.44.6.375
- Author:
Kyung Chul CHOI
1
;
Do Sung YOO
;
Kyung Sock CHO
;
Pil Woo HUH
;
Dal Soo KIM
;
Chun Kun PARK
Author Information
1. Department of Neurosurgery, Uijeongbu St. Mary's Hospital, The Catholic University of Korea College of Medicine, Uijeongbu, Korea. yooman@catholic.ac.kr
- Publication Type:Original Article
- Keywords:
Neuron;
Single growth factor;
Combination of growth factors;
Synergistic effect
- MeSH:
Animals;
Brain-Derived Neurotrophic Factor;
Fibroblast Growth Factor 2;
Insulin;
Insulin-Like Growth Factor I;
Intercellular Signaling Peptides and Proteins;
Nerve Growth Factor;
Neural Stem Cells;
Neurons;
Rats;
Tubulin
- From:Journal of Korean Neurosurgical Society
2008;44(6):375-381
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVE: The effects on neural proliferation and differentiation of neural stem cells (NSC) of basic fibroblast growth factor-2 (bFGF), insulin growth factor-I (IGF-I), brain-derived neurotrophic factor (BDNF), and nerve growth factor (NGF) were assessed. Also, following combinations of various factors were investigated : bFGF+IGF-I, bFGF+BDNF, bFGF+NGF, IGF-I+BDNF, IGF-I+NGF, and BDNF+NGF. METHODS: Isolated NSC of Fisher 344 rats were cultured with individual growth factors, combinations of factors, and no growth factor (control) for 14 days. A proportion of neurons was analyzed using beta-tubulin III and NeuN as neural markers. RESULTS: Neural differentiations in the presence of individual growth factors for beta-tubulin III-positive cells were : BDNF, 35.3%; IGF-I, 30.9%; bFGF, 18.1%; and NGF, 15.1%, and for NeuN-positive cells was : BDNF, 34.3%; bFGF, 32.2%; IGF-1, 26.6%; and NGF, 24.9%. However, neural differentiations in the absence of growth factor was only 2.6% for beta-tubulin III and 3.1% for NeuN. For beta-tubulin III-positive cells, neural differentiations were evident for the growth factor combinations as follows : bFGF+IGF-I, 73.1%; bFGF+NGF, 65.4%; bFGF+BDNF, 58.7%; BDNF+IGF-I, 52.2%; NGF+IGF-I, 40.6%; and BDNF+NGF, 40.0%. For NeuN-positive cells : bFGF+IGF-I, 81.9%; bFGF+NGF, 63.5%; bFGF+BDNF, 62.8%; NGF+IGF-I, 62.3%; BDNF+NGF, 56.3%; and BDNF+IGF-I, 46.0%. Significant differences in neural differentiation were evident for single growth factor and combination of growth factors respectively (p<0.05). CONCLUSION: Combinations of growth factors have an additive effect on neural differentiation. The most prominent neural differentiation results from growth factor combinations involving bFGF and IGF-I. These findings suggest that the combination of a mitogenic action of bFGF and postmitotic differentiation action of IGF-I synergistically affects neural proliferation and NSC differentiation.
